Peripheral demyelinating diseases entail damage to axons and Schwann cells in the peripheral nervous system. Because of poor prognosis and lack of a cure, this group of diseases has a global impact. The primary underlying cause of these diseases involves the inability of Schwann cells to remyelinate the damaged insulating myelin around axons, resulting in neuronal death over time. In the past decade, extensive research has been directed in the direction of Schwann cells focusing on their physiological and neuroprotective effects on the neurons in the peripheral nervous system. One cause of dysregulation in the remyelinating function of Schwann cells has been associated with oxidative stress. Tissue-engineered biodegradable scaffolds that can stimulate remyelination response in Schwann cells have been proposed as a potential treatment strategy for peripheral demyelinating diseases. However, strategies developed to date primarily focussed on either remyelination or oxidative stress in isolation. Here, we have developed a multifunctional nanofibrous scaffold with material and biochemical cues to tackle both remyelination and oxidative stress in one matrix. We developed a nanofibrous scaffold using polycaprolactone (PCL) as a foundation loaded with antioxidant graphene oxide (GO) and coated this bioscaffold with Schwann cell acellular matrix. In vitro studies revealed both antioxidant and remyelination properties of the developed bioscaffold. Based on the results, the developed multifunctional bioscaffold approach can be a promising biomaterial approach for treating demyelinating diseases.

Graphical Abstract

中文翻译：

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具有抗氧化和早髓鞘形成功能的聚己内酯/氧化石墨烯/脱细胞基质纳米纤维支架用于治疗外周脱髓鞘疾病

周围脱髓鞘疾病会损害周围神经系统中的轴突和雪旺细胞。由于预后不良且缺乏治愈方法，这组疾病具有全球影响。这些疾病的根本原因是雪旺细胞无法对轴突周围受损的绝缘髓鞘进行髓鞘再生，从而导致神经元随着时间的推移而死亡。在过去的十年中，广泛的研究集中在施万细胞的方向上，重点关注其对周围神经系统神经元的生理和神经保护作用。雪旺细胞髓鞘再生功能失调的原因之一与氧化应激有关。可以刺激雪旺细胞髓鞘再生反应的组织工程生物可降解支架已被提议作为外周脱髓鞘疾病的潜在治疗策略。然而，迄今为止制定的策略主要集中于髓鞘再生或单独的氧化应激。在这里，我们开发了一种多功能纳米纤维支架，具有材料和生化线索，可以解决一个基质中的髓鞘再生和氧化应激问题。我们开发了一种纳米纤维支架，使用聚己内酯（PCL）作为负载抗氧化剂氧化石墨烯（GO）的基础，并用雪旺细胞非细胞基质涂覆该生物支架。体外研究揭示了所开发的生物支架的抗氧化和髓鞘再生特性。根据这些结果，所开发的多功能生物支架方法可能成为治疗脱髓鞘疾病的一种有前途的生物材料方法。

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