The use of beneficial organisms for the biocontrol of soil-borne pathogens in forestry is still poor explored. In this work, the nutritional demands of 10 previously selected isolates of Trichoderma for the biocontrol of forest soil-borne pathogens have been tested by Phenotype Microarray technology, to investigate about their C-source utilization and exploring the possibility to obtain a microbial consortia (SynCom), an innovative strategy for the biocontrol of plant disease. All Trichoderma isolates tested in this study showed a high spore germination percentage within 3 d and evidenced nutritional preference regardless of the species they belong to, and unrelated to their soil of origin. Results of growth curve analysis and MANOVA test revealed that all isolates assimilate a broad range of substrates, generally preferring complex compounds such as monosaccharides related compounds, nitrogen compounds, carboxylic acids and esters. No evidence of competition for nutritional resources have been observed among isolates of this study. As a result, a combination of different isolates could be proposed to obtain a SynCom useful for the practice of phytopathogen biocontrol in forestry. The addition of i-erythritol, adenosine and turanose to a growth substrate could be suggested as stimulating compounds for the growth of the selected Trichoderma isolates.

利用有益生物对林业土传病原体进行生物防治的探索仍然很少。在这项工作中，通过表型微阵列技术测试了 10 个先前选择的用于森林土传病原体生物防治的木霉分离株的营养需求，以调查其碳源利用并探索获得微生物群落（SynCom）的可能性。 ），植物病害生物防治的创新策略。本研究中测试的所有木霉分离株均在 3 天内显示出较高的孢子萌发率，并且无论其所属物种如何，都表现出营养偏好，并且与其来源土壤无关。生长曲线分析和多元方差分析测试的结果表明，所有分离物都同化了广泛的底物，通常更喜欢复杂的化合物，例如单糖相关化合物、氮化合物、羧酸和酯。本研究的分离株之间没有观察到营养资源竞争的证据。因此，可以建议将不同分离株组合以获得可用于林业植物病原体生物防治实践的 SynCom。可以建议向生长底物中添加异赤藓糖醇、腺苷和松二糖作为所选木霉分离株生长的刺激化合物。