Situated in the oral cavity, the rabbit palatine tonsils are part of the mucosal immune system and help to defend the body against foreign pathogens. Expressed as two oval protrusions in the wall of the oropharynx, the rabbit palatine tonsils are characterized by excretory ducts and trabeculae. We here compare paraffin embedded and cryosections of the healthy rabbit tonsils. This analysis centers on evaluating the differential outcomes resulting from the application of these fixation methodologies in conjunction with immunohistochemical assays targeting collagen I, collagen III, fibronectin, α-smooth muscle actin (α-SMA), and ki67. Subsequent recommendations are provided based on our findings. Furthermore, we demonstrate the advantage of an antigen retrieval step in immunohistochemical labeling of paraffin sections. Basic classical histological stainings as HE, GT and elastin were also performed. Comparison of different stainings and labelings was furthermore performed in serial sections, showing that adjacent to the excretory ducts, the tonsillar tissue was particularly composed of collagen I and fibronectin, while the vessel walls were predominantly α-SMA positive. Moreover, PAR-2 immunohistochemical staining was performed, where a small fraction of the cells found in the tonsillar connective tissue were PAR-2 positive (probably a subpopulation of mast cells), as well as the lumen of some excretory ducts and trabeculae. Collagen III on the other hand was only weakly expressed in the tonsils. Proliferating ki67 positive cells were rare. This endeavor serves to furnish the scientific community with reference imagery pertinent to researchers opting for the rabbit palatine tonsil model. The diversity of staining techniques employed herein establishes a foundational repository of images, primed for comparative analysis against pathological conditions. Furthermore, these images hold the potential to illustrate inter-species variations. For instance, they can be juxtaposed against murine or rodent tonsils, or even offer insights into the human context.

兔子的腭扁桃体位于口腔中，是粘膜免疫系统的一部分，有助于保护身体免受外来病原体的侵害。兔腭扁桃体表现为口咽壁上的两个椭圆形突起，其特征是排泄管和小梁。我们在这里比较健康兔子扁桃体的石蜡包埋和冷冻切片。该分析的重点是评估应用这些固定方法与针对胶原蛋白 I、胶原蛋白 III、纤连蛋白、α-平滑肌肌动蛋白 (α-SMA) 和 ki67 的免疫组织化学测定所产生的差异结果。根据我们的发现提供了后续建议。此外，我们证明了石蜡切片免疫组织化学标记中抗原修复步骤的优势。还进行了基本的经典组织学染色，如 HE、GT 和弹性蛋白。此外，在连续切片中对不同染色和标记进行比较，结果表明，邻近排泄管的扁桃体组织特别由 I 型胶原蛋白和纤连蛋白组成，而血管壁主要为 α-SMA 阳性。此外，进行了 PAR-2 免疫组织化学染色，发现扁桃体结缔组织中发现的一小部分细胞呈 PAR-2 阳性（可能是肥大细胞的亚群），以及一些排泄管和小梁的管腔。另一方面，III 型胶原蛋白仅在扁桃体中微弱表达。增殖的 ki67 阳性细胞很少见。这项努力旨在为科学界提供与选择兔子腭扁桃体模型的研究人员相关的参考图像。本文采用的染色技术的多样性建立了图像的基础存储库，为针对病理条件的比较分析做好了准备。此外，这些图像有可能说明物种间的差异。例如，它们可以与鼠类或啮齿动物扁桃体并列，甚至可以提供对人类背景的见解。