Stem cells produce nanosized particles known as extracellular vesicles (SC-EVs), which therapeutically affect stem cells. EVs are more abundantly produced, exhibit better stability, and possess lower immune rejection rates than stem cells. However, the traditional methods of isolating EVs, such as ultracentrifugation, possess limitations that require a complex process and consume more time. Moreover, it is difficult to isolate specific EVs that have target surface proteins that affect regenerative effects. To address these limitations, a new dual-mode horseshoe-shaped orifice micromixer (DM-HOMM) chip that can bind antibody-conjugated micromagnetic beads and SC-EVs and sequentially elute specific SC-EVs on the beads using an eluent was developed. For effective elution from the microbead-SC-EV complex, four types of eluents were used to control pH and ionic strength between antibodies and surface proteins in EVs. In addition, we investigated the reusability of antibody-conjugated micromagnetic beads. The beads indicated identical binding efficiencies between the antibodies and specific SC-EVs for three repeated cycles using the dual-mode chip. CD63⁺ EVs collected by the chip exhibited higher cell viability and regeneration effects than untreated and total EVs. This SC-EVs’ isolation method possesses the potential for targeted therapeutic applications and enhanced regenerative effects.

干细胞产生被称为细胞外囊泡（SC-EV）的纳米颗粒，它对干细胞有治疗作用。与干细胞相比，EV 的产量更丰富，稳定性更好，免疫排斥率更低。然而，分离 EV 的传统方法（例如超速离心）具有局限性，需要复杂的过程并消耗更多的时间。此外，很难分离出具有影响再生效果的目标表面蛋白的特定电动汽车。为了解决这些限制，开发了一种新型双模式马蹄形孔口微混合器 (DM-HOMM) 芯片，该芯片可以结合抗体偶联的微磁珠和 SC-EV，并使用洗脱液依次洗脱磁珠上的特定 SC-EV。为了从微珠-SC-EV 复合物中有效洗脱，使用四种类型的洗脱液来控制 EV 中抗体和表面蛋白之间的 pH 值和离子强度。此外，我们还研究了抗体偶联微磁珠的可重复使用性。使用双模式芯片进行三个重复循环后，珠子表明抗体和特定 SC-EV 之间的结合效率相同。CD63⁺芯片收集的 EV 比未经处理的 EV 和总 EV 表现出更高的细胞活力和再生效果。这种 SC-EV 的隔离方法具有靶向治疗应用和增强再生效果的潜力。