Specialized pro-resolving lipid mediators (SPMs), derived from polyunsaturated fatty acids are important mediators in the resolution of inflammation. Recent studies have focused on the effects of SPMs in cardiovascular health and diseases. However, little is known about the effect SPMs on human vascular tone. Therefore, in this study it is aimed to investigate the effect of various SPMs including resolvin D- and E-series, maresin-1 (MaR1) and lipoxin-A4 (LxA4) on the vascular tone of human isolated saphenous vein (SV) preparations under inflammatory conditions. In addition, we aimed to evaluate the effects of SPMs on the release of pro-inflammatory mediators, monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF- α) from human SV. Pretreatment of isolated of human SV with resolvin E1 (RvE1), resolvin D1 (RvD1) and MaR1 (100 nM, 18 h) significantly reduced the contractile responses to thromboxane A2 mimetic, U46619 whereas pretreatment with LxA4 and RvD2 (100 nM, 18 h) had no significant effect on the vascular tone of SV. Moreover, RvE1, RvD1 and MaR1 but not LxA4 and RvD2 (100 nM, 18 h) pretreatment diminished the release of MCP-1 and TNF-α from SV. In conclusion, our findings suggest that pre-treatment with RvE1, RvD1, and MaR1 could have potential benefits in decreasing graft vasospasm and vascular inflammation in SV.

源自多不饱和脂肪酸的专门促消退脂质介质 (SPM) 是消退炎症的重要介质。最近的研究重点关注 SPM 对心血管健康和疾病的影响。然而，关于 SPM 对人体血管张力的影响却知之甚少。因此，本研究旨在研究各种 SPM（包括 resolvin D 系列和 E 系列、maresin-1 (MaR1) 和 lipoxin-A4 (LxA4)）对人离体隐静脉 (SV) 制剂血管张力的影响在炎症条件下。此外，我们的目的是评估 SPM 对人 SV 释放促炎介质、单核细胞趋化蛋白-1 (MCP-1) 和肿瘤坏死因子-α (TNF-α) 的影响。用 resolvin E1 (RvE1)、resolvin D1 (RvD1) 和 MaR1（100 nM，18 小时）预处理分离的人 SV 显着降低对血栓素 A2 模拟物 U46619 的收缩反应，而用 LxA4 和 RvD2（100 nM，18 小时）预处理）对 SV 的血管张力没有显着影响。此外，RvE1、RvD1 和 MaR1 但 LxA4 和 RvD2（100 nM，18 小时）预处理减少了 SV 中 MCP-1 和 TNF-α 的释放。总之，我们的研究结果表明，RvE1、RvD1 和 MaR1 预处理可能具有减少 SV 移植物血管痉挛和血管炎症的潜在益处。