The growth of shrimp production through aquaculture has led to efforts to optimize processes such as nutrition during the prematuration and reproductive phases. This study sought an optimal culture system and diet to enhance the reproductive characteristics of male Litopenaeus vannamei during prematuration: this compared biofloc with clear water, and an experimental pellet (EP) with a mix of fresh food. Effects were measured in terms of shrimp survival, sperm quality, immunological parameters including prophenoloxidase (ProPO) and hemocyte count, nutritional condition measured as metabolites in hemolymph, hepatopancreas, and reproductive tract, oxidative stress biomarkers in the hepatopancreas and reproductive tract, and relative expression of superoxide dismutase, catalase, glutathione peroxidase, ProPO, alpha2-macroglobulin and caspase in reproductive tract. Survival was higher with pellets (100%) than with fresh food (87%). Shrimp that were fed pellets had a higher hepatosomatic index, higher nutrient concentration in the hepatopancreas, and a higher antioxidant activity in the reproductive tract. Notably, sperm quality remained similar regardless of culture system or diet. These promising results should be ratified with a bioeconomic study to check the feasibility of hatcheries to use monosex tanks and feeding the males with EPs.

中文翻译：

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两种培养系统中新鲜或实验食物喂养的凡纳滨对虾的精子质量

通过水产养殖实现对虾产量的增长导致人们努力优化虾类生产过程，例如在成熟前和繁殖阶段的营养。本研究寻求最佳的培养系统和饮食，以增强雄性凡纳滨对虾在预成熟期间的繁殖特性：该研究将生物絮团与清水以及实验颗粒（EP）与新鲜食物的混合物进行了比较。影响的测量包括虾的存活率、精子质量、免疫学参数（包括原酚氧化酶（ProPO）和血细胞计数）、以血淋巴、肝胰腺和生殖道中的代谢物测量的营养状况、肝胰腺和生殖道中的氧化应激生物标志物以及相对表达生殖道中的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、ProPO、α2-巨球蛋白和半胱天冬酶。颗粒食品的存活率 (100%) 高于新鲜食品 (87%)。饲喂颗粒饲料的虾具有较高的肝体指数、肝胰腺的营养浓度较高以及生殖道的抗氧化活性较高。值得注意的是，无论培养系统或饮食如何，精子质量都保持相似。这些有希望的结果应该得到生物经济学研究的认可，以检查孵化场使用单性水箱并用 EP 喂养雄性的可行性。