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Responses of digestive enzyme profiles in newly-hatched (Zoea I) larvae of the mud crab Scylla serrata to intermittent food availability and food deprivation
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 2.2 ) Pub Date : 2023-10-08 , DOI: 10.1016/j.cbpb.2023.110906
Jerome Genodepa 1 , Chaoshu Zeng 1 , Thane A Militz 2 , Paul C Southgate 2
Affiliation  

Activities (mU larva−1) of enzymes critical to digestion were examined to better understand how newly-hatched (Zoea I) larvae of the mud crab Scylla serrata respond to intermittent food availability and food deprivation. Specifically, this study examined the activities of trypsin-like proteases, nonspecific esterases, and α-amylase across three experiments that simulated scenarios in which larvae hatch and experience rearing conditions where food was either: (1) continuously available or unavailable; (2) initially unavailable, but subsequently available; or (3) initially available, but subsequently unavailable. Results showed that food availability exerts a significant influence on enzyme profiles in newly-hatched larvae, with nutritional history influencing their response to food deprivation. When food was unavailable from hatch, there was no significant change in larval enzyme activities between 6 and 78 h post-hatch. If food became available at any point during this period, however, newly-hatched larvae were capable of rapidly (within 12–24 h) adjusting enzyme activities in response. Furthermore, a short (36 h) period of food availability appears sufficient to permit continuous substrate utilization during subsequent food deprivation of equivalent duration. Such flexibility is an important physiological strategy allowing newly-hatched larvae of S. serrata to adapt and thrive in challenging tropical oceanic environments and provides a basis for optimizing protocols for hatchery production of this species.



中文翻译:

锯缘青蟹新孵化 (Zoea I) 幼虫消化酶谱对间歇性食物供应和食物匮乏的反应

检查对消化至关重要的酶的活性(mU larva -1 ),以更好地了解锯缘青蟹新孵化(Zoea I)幼虫如何应对间歇性食物供应和食物匮乏。具体来说,这项研究通过三个实验检查了胰蛋白酶样蛋白酶、非特异性酯酶和 α-淀粉酶的活性,这些实验模拟了幼虫孵化和经历食物以下情况的饲养条件:(1)持续可用或不可用;(2) 最初不可用,但随后可用;(3) 最初可用,但随后不可用。结果表明,食物供应对新孵化幼虫的酶谱有显着影响,营养史影响它们对食物匮乏的反应。当孵化后无法获得食物时,孵化后 6 至 78 小时内幼虫酶活性没有显着变化。然而,如果在此期间的任何时候有食物可用,新孵化的幼虫都能够快速(12-24 小时内)调整酶活性以做出反应。此外,短时间(36小时)的食物供应似乎足以允许在随后同等时间的食物匮乏期间连续利用基质。这种灵活性是一种重要的生理策略,使刚孵化的锯齿鱼幼虫能够在具有挑战性的热带海洋环境中适应和繁衍生息,并为优化该物种孵化场生产方案提供了基础。

更新日期:2023-10-11
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