Acute gastroenteritis (AGE) in children represents a health problem. Besides common enteric viruses such as rotavirus and adenovirus, new viruses such as cosavirus, salivirus, and bufavirus may be associated with AGE. The objective of the study was to detect cosavirus, salivirus, and bufavirus viruses in children below 5 years with acute gastroenteritis by the use of real-time polymerase chain reaction (PCR) besides detection of rotavirus and adenovirus by enzyme-linked immunosorbent assay (ELISA). The study included 150 children ≤ 5 years with community-acquired diarrhea. Stool samples from children were subjected for the detection of rotavirus and adenovirus antigens by ELISA and for detection of buvavirus, salivirus, and cosavirus by real-time PCR. The commonest virus detected in the stool samples of children with AGE was rotavirus 31.3% followed by adenovirus 24%. Among the new viruses studied, salivirus was detected in six samples (4.0%), buvavirus was detected in four samples (2.7%), and cosavirus was detected in two samples (1.3%). The mixed rotavirus detection with studied viruses was 23.4% for adenovirus, 4.3% for calicivirus, and 2.1% for bocavirus, and none of the detected cosavirus was associated with rotavirus. In the studied children, at least one of the new viruses was detected in ten children (6.7%). Buvavirus, salivirus, and cosavirus were detected as a single virus (0.7%) in the children with acute gastroenteritis and buvavirus was detected with cosavirus without other viruses in one sample (0.7%). The study reports the occurrence of buvavirus, cosavirus, and salivirus in the pediatric patients with community-acquired acute gastroenteritis. There was a high prevalence of rotavirus and adenovirus antigens in those patients with low positivity for buvavirus, cosavirus, and salivirus viruses. There is a need for a large cohort study to study the prevalence of buvavirus, cosavirus, and salivirus in pediatrics with acute gastroenteritis and to validate their association with the disease.

中文翻译：

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小儿急性胃肠炎中 Cosavirus、Salivirus 和 Bufavirus 病毒的研究

儿童急性胃肠炎（AGE）是一个健康问题。除了轮状病毒、腺病毒等常见肠道病毒外，糖病毒、唾液病毒、蟾蜍病毒等新病毒也可能与AGE有关。该研究的目的是除了通过酶联免疫吸附试验（ELISA）检测轮状病毒和腺病毒外，还通过实时聚合酶链反应（PCR）检测5岁以下急性胃肠炎儿童中的衣原体病毒、唾液病毒和蟾蜍病毒。 ）。该研究纳入了 150 名 5 岁以下患有社区获得性腹泻的儿童。通过 ELISA 检测儿童粪便样本的轮状病毒和腺病毒抗原，并通过实时 PCR 检测布瓦病毒、唾液病毒和 cosavirus。在 AGE 儿童粪便样本中检测到的最常见病毒是轮状病毒 31。3% 其次是腺病毒 24%。在研究的新病毒中，在 6 个样本中检测到唾液病毒（4.0%），在 4 个样本中检测到布瓦病毒（2.7%），在 2 个样本中检测到 cosavirus（1.3%）。与研究病毒混合的轮状病毒检出率为：腺病毒为 23.4%，杯状病毒为 4.3%，博卡病毒为 2.1%，检测到的 Cosavirus 均与轮状病毒无关。在所研究的儿童中，有 10 名儿童（6.7%）至少检测到一种新病毒。急性胃肠炎患儿中布瓦病毒、唾液病毒和衣索病毒为单一病毒（0.7%），而布瓦病毒在一份样本中与衣索病毒一起检出，没有其他病毒（0.7%）。该研究报告了社区获得性急性胃肠炎儿童患者中布瓦病毒、衣索病毒和唾液病毒的发生情况。在布瓦病毒、冠状病毒和唾液病毒呈低阳性的患者中，轮状病毒和腺病毒抗原的患病率较高。需要进行大型队列研究来研究布瓦病毒、衣病毒和唾液病毒在患有急性胃肠炎的儿科中的流行情况，并验证它们与该疾病的关联。