is a gram-positive, anaerobic sporulating bacillus which can infect several hosts, thereby being considered the causative agent of many gut illnesses. Some studies have suggested that ’s virulence factors may negatively affect gut microbiota homeostasis by decreasing beneficial bacteria; however, studies have failed to evaluate the simultaneous presence of other pathogenic bacteria, such as (another sporulating bacillus known to play a role in gut microbiota imbalance). Conscious of the lack of compelling data, this work has ascertained how such microorganisms’ coexistence can be associated with a variation in gut microbiota composition, compared to that of colonisation. PCR was thus used for identifying and in 98 samples. Amplicon-based sequencing of 16S- and 18S-rRNA genes’ V4 hypervariable region from such samples was used for determining the microbiota’s taxonomical composition and diversity. Small differences were observed in bacterial communities’ taxonomic composition and diversity; such imbalance was mainly associated with groups having hospital-acquired diarrhoea. The alterations reported herein may have been influenced by and diarrhoea acquisition site, despite ’ ability to cause alterations in microbiota due to its virulence factors Our findings highlight the need for a holistic view of gut microbiota.

中文翻译：

---

基于采集位点的产气荚膜梭菌和艰难梭菌相关肠道微生物群的重塑

是一种革兰氏阳性厌氧芽孢杆菌，可以感染多种宿主，因此被认为是许多肠道疾病的病原体。一些研究表明，大肠杆菌的毒力因子可能会减少有益细菌，从而对肠道微生物群稳态产生负面影响。然而，研究未能评估其他致病菌的同时存在，例如（另一种已知在肠道微生物群失衡中发挥作用的芽孢杆菌）。由于缺乏令人信服的数据，这项工作已经确定了与定殖相比，这些微生物的共存如何与肠道微生物群组成的变化相关。因此，使用 PCR 来鉴定 98 个样品。对此类样本中的 16S-和 18S-rRNA 基因 V4 高变区进行基于扩增子的测序，用于确定微生物群的分类组成和多样性。细菌群落的分类组成和多样性存在微小差异；这种不平衡主要与患有医院获得性腹泻的群体有关。本文报道的变化可能受到腹泻采集位点的影响，尽管其毒力因素能够引起微生物群的改变。我们的研究结果强调了对肠道微生物群进行整体观察的必要性。