Reproducibility in animal research is impacted by the environment, by husbandry practices in the laboratory and by the animals' provenance. These factors, however, are often not adequately considered by researchers. A disconnect between researchers and animal care staff can result in inappropriate housing and husbandry decisions for scientific studies with those animals. This is especially the case for the research in neuro-behaviour, epigenetics, and the impact of climate change, as heritable phenotypic, behavioural or physiological changes are known to result from the animals' environmental housing, husbandry, provenance and prior experience. This can lead to greater variation (even major differences) in data outcomes among studies, driving scientific uncertainties. Herein, we illustrate some of the endpoints measured in fish studies known to be intrinsically linked to the environment and husbandry conditions and assess the significance of housing and husbandry practice decisions for research adopting these endpoints for different fish species. We highlight the different priorities and challenges faced by researchers and animal care staff and how harmonising their activities and building greater understanding of how husbandry practices affect the fish will improve reproducibility in research outcomes. We furthermore illustrate how improving engagement between stakeholders, including regulatory bodies, can better underpin fish husbandry decisions and where researchers could help to drive best husbandry practices through their own research with fish models.

中文翻译：

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超越合规性：协调研究和饲养实践，以提高使用鱼类模型的实验再现性

动物研究的可重复性受到环境、实验室饲养方法和动物来源的影响。然而，研究人员往往没有充分考虑这些因素。研究人员和动物护理人员之间的脱节可能会导致对这些动物进行科学研究时做出不适当的饲养和饲养决定。对于神经行为、表观遗传学和气候变化影响的研究尤其如此，因为已知可遗传的表型、行为或生理变化是由动物的环境住房、饲养、起源和先前的经验造成的。这可能会导致研究之间的数据结果出现更大的差异（甚至重大差异），从而增加科学的不确定性。在此，我们说明了鱼类研究中测量的一些已知与环境和饲养条件有内在联系的终点，并评估了住房和饲养实践决策对于对不同鱼类采用这些终点的研究的重要性。我们强调研究人员和动物护理人员面临的不同优先事项和挑战，以及协调他们的活动并更好地了解饲养方法如何影响鱼类将如何提高研究结果的可重复性。我们还说明了如何改善包括监管机构在内的利益相关者之间的参与，可以更好地支持鱼类养殖决策，以及研究人员可以通过自己对鱼类模型的研究来帮助推动最佳养殖实践。