Drosophila imaginal disc cells can change their identity under stress conditions through transdetermination (TD). Research on TD can help elucidate the in vivo process of cell fate conversion. We previously showed that the overexpression of winged eye (wge) induces eye-to-wing TD in the eye disc and that an insulin-like peptide, Dilp8, is then highly expressed in the disc. Although Dilp8 is known to mediate systemic developmental delay via the Lgr3 receptor, its role in TD remains unknown. This study showed that Dilp8 is expressed in specific cells that do not express eye or wing fate markers during Wge-mediated TD and that the loss of Dilp8 impairs the process of eye-to-wing transition. Thus, Dilp8 plays a pivotal role in the cell fate conversion under wge overexpression. Furthermore, we found that instead of Lgr3, another candidate receptor, Drl, is involved in Wge-mediated TD and acts locally in the eye disc cells. We propose a model in which Dilp8–Drl signaling organizes cell fate conversion in the imaginal disc during TD.

中文翻译：

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Dilp8 及其候选受体 Drl 参与果蝇成虫盘的转决定

果蝇成虫盘细胞可以在应激条件下通过转决定（TD）改变其身份。对TD的研究有助于阐明细胞命运转换的体内过程。我们之前表明，翼状眼( wge )的过度表达会诱导眼盘中的眼对翼 TD，然后胰岛素样肽 Dilp8 在眼盘中高表达。尽管 Dilp8 已知可通过 Lgr3 受体介导系统性发育迟缓，但其在 TD 中的作用仍不清楚。这项研究表明，在 Wge 介导的 TD 过程中，Dilp8 在不表达眼睛或翅膀命运标记的特定细胞中表达，并且 Dilp8 的丢失会损害眼睛到翅膀的过渡过程。因此，Dilp8在wge过表达下的细胞命运转换中发挥着关键作用。此外，我们发现另一种候选受体 Drl 代替 Lgr3 参与 Wge 介导的 TD，并在眼盘细胞中局部发挥作用。我们提出了一个模型，其中 Dilp8-Drl 信号传导在 TD 期间组织成虫盘中的细胞命运转换。