The color is the primal property of the objects around us and is direct manifestation of light-matter interactions. The color information is used in many different fields of science, technology and industry to investigate material properties or for identification of concentrations of substances. Usually the color information is used as a global parameter in a macro scale. To quantitatively measure color information in micro scale one needs to use dedicated microscope spectrophotometers or specialized micro-reflectance setups. Here, the Colorimetric Microscopy (C-Microscopy) approach based on digital optical microscopy and a free software is presented. The C-Microscopy approach uses color calibrated image and colorimetric calculations to obtain physically meaningful quantities i.e., dominant wavelength and excitation purity maps at micro level scale. This allows for the discovery of the local color details of samples surfaces. Later, to fully characterize the optical properties, the hyperspectral reflectance data at micro scale (reflectance as a function of wavelength for a each point) are colorimetrically recovered. The C-Microscopy approach was successfully applied to various types of samples i.e., two metamorphic rocks unakite and lapis lazuli, which are mixtures of different minerals; and to the surface of gold 99.999 % pellet, which exhibits different types of surface features. The C-Microscopy approach could be used to quantify the local optical properties changes of various materials at microscale in an accessible way. The approach is freely available as a set of python jupyter notebooks.

颜色是我们周围物体的原始属性，是光与物质相互作用的直接表现。颜色信息用于科学、技术和工业的许多不同领域，以研究材料特性或识别物质的浓度。通常颜色信息被用作宏观尺度的全局参数。为了定量测量微观尺度的颜色信息，需要使用专用的显微镜​​分光光度计或专门的微反射装置。这里介绍了基于数字光学显微镜和免费软件的比色显微镜（C-显微镜）方法。C-显微镜方法使用颜色校准图像和比色计算来获得物理上有意义的量，即微观尺度上的主波长和激发纯度图。这允许发现样本表面的局部颜色细节。随后，为了充分表征光学特性，通过比色恢复微观尺度的高光谱反射率数据（反射率是每个点波长的函数）。C 显微镜方法已成功应用于各种类型的样品，即两种变质岩乌凯特石和青金石，它们是不同矿物的混合物；以及 99.999% 金颗粒的表面，其呈现出不同类型的表面特征。C-显微镜方法可用于以一种易于理解的方式量化微尺度上各种材料的局部光学特性变化。该方法可以作为一组 python jupyter 笔记本免费提供。