Backgrounds

With an increase in the world’s aging population, candidate substances for the management of neurodegenerative diseases, including Alzheimer’s disease (AD), have received considerable attention. Amyloid beta (Aβ) peptide, the leading cause of AD, induces synaptic deficits, eventually leading to cognitive impairment. Plasmalogen is a subclass of glycerophospholipids containing a vinyl ether group in the glycerol backbone which has various efficacies.

Objectives

In this study, we investigated the mechanisms of scallop-derived plasmalogen (SPL) in neurotoxicity in human neurons, which remain incompletely understood. SH-SY5Y human neuroblastaoma cells were treated with Aβ 1–42, a major component of amyloid deposits in AD brains.

Results

SPL inhibited the phosphorylation of glycogen synthase kinase 3β and tau induced by Aβ. In addition, SPL decreased Aβ generation by downregulating the expression of amyloid precursor protein (APP) and beta-site APP cleaving enzyme 1. Furthermore, SPL suppressed the release of pro-inflammatory cytokines and expression of apoptotic proteins induced by Aβ.

Conclusion

Our study indicates that SPL would reduce Aβ-induced neurotoxicity associated with inflammation and apoptosis in human neuroblastoma cells.

中文翻译：

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扇贝缩醛磷脂可减轻β淀粉样蛋白诱导的 SH-SY5Y 细胞炎症和细胞凋亡

背景

随着世界人口老龄化的增加，用于治疗神经退行性疾病（包括阿尔茨海默氏病（AD））的候选物质受到了相当大的关注。β 淀粉样蛋白 (Aβ) 肽是 AD 的主要原因，可引起突触缺陷，最终导致认知障碍。缩醛磷脂是甘油磷脂的一个亚类，其甘油主链中含有乙烯基醚基团，具有多种功效。

目标

在这项研究中，我们研究了扇贝缩醛磷脂（SPL）对人类神经元的神经毒性的机制，目前该机制尚不完全清楚。SH-SY5Y 人神经母细胞瘤细胞用 Aβ 1-42 处理，Aβ 1-42 是 AD 大脑中淀粉样蛋白沉积物的主要成分。

结果

SPL 抑制 Aβ 诱导的糖原合酶激酶 3β 和 tau 磷酸化。此外，SPL 通过下调淀粉样前体蛋白 (APP) 和 β 位点 APP 裂解酶 1 的表达来减少 Aβ 生成。此外，SPL 还抑制 Aβ 诱导的促炎细胞因子的释放和凋亡蛋白的表达。

结论

我们的研究表明，SPL 可以减少 Aβ 诱导的与人神经母细胞瘤细胞炎症和凋亡相关的神经毒性。