The present study focuses on quantitative phase imaging of erythrocytes with the aim to evaluate the effects of high-dose methylprednisolone (HDMP) on erythrocytes in vivo under physiological conditions in human blood samples. Samples from ten patients, prescribed to be treated with 1000 mg/day intravenous methylprednisolone for 5 days, were analyzed by white light diffraction phase microscopy (WDPM) for quantitative imaging. WDPM, an optical measurement technique, enables single shot measurement and low speckle noise using white light. Quantitative phase imaging performed with this experimental setup allowed the determination of erythrocyte morphology with 9 different parameters. In vivo quantitative analysis of erythrocytes by WDPM, which is a simple and reliable method, shows that HDMP treatment has no significant effect on erythrocyte morphology. With the developing technology, interdisciplinary studies on individuals under treatment should play an important role in elucidating the interaction between steroids and erythrocytes.

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研究高剂量甲基强的松龙对红细胞形态影响的新方法：白光衍射显微镜

本研究重点关注红细胞的定量相位成像，旨在评估生理条件下人体血液样本中高剂量甲基强的松龙（HDMP）对体内红细胞的影响。来自 10 名患者的样本，按照处方接受 1000 毫克/天静脉注射甲泼尼龙治疗 5 天，通过白光衍射相位显微镜 (WDPM) 进行分析，以进行定量成像。WDPM 是一种光学测量技术，可使用白光实现单次测量和低散斑噪声。使用该实验装置进行的定量相位成像可以确定具有 9 个不同参数的红细胞形态。WDPM对红细胞的体内定量分析是一种简单可靠的方法，表明HDMP处理对红细胞形态没有明显影响。随着技术的发展，对接受治疗的个体进行跨学科研究应该在阐明类固醇和红细胞之间的相互作用方面发挥重要作用。