The current study describes the design, analytical characterization, and testing of aptamer sensors based on Si nanonets (Si NN) for thrombin electrochemical detection. Si nanonets are thin sheet arrays of randomly aligned Si nanowires with controlled and programmable properties that may be transported and integrated on a variety of substrates. Si nanonets were placed onto a commercial glassy carbon working electrode to create our aptasensor. Then they were functionalized by conductive pyrrole polymerization, which was followed by aptamer bioreceptor grafting. Electrochemical detection of thrombin was carried out after optimizing the electrode properties. On the one hand, cyclic voltammetry analyses demonstrated a linear association between the acquired current and the logarithm of thrombin concentration from 5 nmol L⁻¹ to 2 µmol L⁻¹. Impedance measurements, on the other hand, revealed a linear relationship between the charge transfer resistance as well as the impedance modulus. Thus, for the first time, Si nanonet thin sheets were used as constitutive nanoporous parts of electrodes capable of detecting thrombin at concentration limits of 5 nM, considered as a risk factor or presence of thrombosis. This demonstrates the concept of using this type of nanomaterial to detect molecules of biological interest.

目前的研究描述了用于凝血酶电化学检测的基于硅纳米网 (Si NN) 的适体传感器的设计、分析表征和测试。硅纳米网是随机排列的硅纳米线的薄片阵列，具有受控和可编程的特性，可以在各种基底上传输和集成。将硅纳米网放置在商用玻碳工作电极上以创建我们的适体传感器。然后通过导电吡咯聚合对它们进行功能化，然后进行适体生物受体接枝。对电极性能进行优化后，进行凝血酶的电化学检测。一方面，循环伏安分析证明了采集的电流与凝血酶浓度从5 nmol L ^-1到2 µmol L ^-1的对数之间存在线性关联。另一方面，阻抗测量揭示了电荷转移电阻与阻抗模量之间的线性关系。因此，硅纳米网薄片首次被用作电极的纳米多孔部分，能够检测浓度极限为 5 nM 的凝血酶，被认为是血栓形成的危险因素或存在。这证明了使用这种类型的纳米材料来检测生物感兴趣的分子的概念。