Depositional profiles often feature sorted layers with stratified porosity and water retention, but the vertical partitioning of CO₂ production and transfer remain unclear. In this study, fine soil and coarse sand were refilled to form three layering patterns: Layer-Mix (soil and sand well-mixed), Layer-Thin (8 thin layers interlaid), and Layer-Thick (4 thick layers interlaid). Three doses of ¹³C-labelled glucose were respectively added to the top, middle, and bottom. The results show that the Layer-Thin and Layer-Thick cumulatively released 62% and 67% less CO₂ than the Layer-Mix. The ¹³C-CO₂ contributed 14.1~60.3% to the total CO₂ released from the Layer-Mix, but was only responsible for 7.3~48.8% of that from the Layer-Thin and 7.0~37.0% of that from the Layer-Thick. The peaks of δ¹³C-CO₂ of the two interlaid columns were lowered and lagged by 1~2 days, but the δ¹³C residue remaining in the soil were on average 3~6‰ more negative than that of the Layer-Mix. The ¹³C-CO₂ contributed more to the total CO₂ when the glucose was added at the top, but the δ¹³C-soil was 3‰ more negative when added at the bottom. Overall, the lagged outgassing and lower share of ¹³C-CO₂ from the two interlaid columns did not match with the more negative ¹³C residue remaining in the soil. Such inconsistency collectively highlights that the interlaid layers did not inhibit the decomposition of ¹³C-labelled glucose (i.e., potentially abundant CO₂ produced), but the low diffusivity of the fine layers significantly impeded CO₂ transfer through the heterogeneously structured soil profile.

_{沉积剖面通常具有具有分层孔隙度和保水性的分选层，但CO 2}产生和转移的垂直分配仍不清楚。本研究中，回填细土和粗砂，形成三种分层模式：混合层（土和沙充分混合）、薄层（8 层薄层交错）和厚层（4 层厚层交错）。顶部、中部和底部分别添加三剂^{13 C标记的葡萄糖。}结果表明，薄层和厚层累计释放的CO ₂比混合层少62%和67%。13 C-CO _{2占Layer-Mix释放的CO 2}总量的14.1~60.3% ^，但仅占Layer-Thin释放的7.3~48.8%和Layer-Mix释放的7.0~37.0%。厚的。^{两个夹层柱的δ 13} C-CO ₂峰值降低并滞后1~2 d，但土壤中残留的δ ¹³ C残留量比层混合层平均负3~6‰ 。当葡萄糖添加在顶部时， 13 C-CO 2 对总CO 2 的贡献更大，但当在底部添加时，δ 13 C-_土壤的_负值增加了3 ^{‰ 。}总体而言，来自两个交错柱的滞后排气和较低份额的¹³ C-CO ₂与土壤中剩余的负^{13 C 残留物不匹配。}这种不一致共同突出表明，夹层不会抑制¹³ C 标记的葡萄糖的分解（即，产生潜在丰富的CO ₂），但细层的低扩散率显着阻碍了CO ₂通过异质结构土壤剖面的转移。