Microbes well-adapted to the Arctic Ocean are promising for producing novel compounds, due to their fancy strategies for adaptation and being under-investigated. Two new phenazine alkaloids (1 and 2) and one new phenoxazine (3) were isolated from Nocardiopsis dassonvillei 502F, a strain originally isolated from Arctic deep-sea sediments. AntiSMASH analysis of the genome of Nocardiopsis dassonvillei 502F revealed the presence of 16 putative biosynthetic gene clusters (BGCs), including a phenazine BGC. Most of the isolated compounds were evaluated for their antibacterial, antiallergic, and cytotoxic activities. Among them, compounds 4 and 5 exhibited potent in vitro cytotoxic activities against osteosarcoma cell line 143B with IC₅₀ values 0.16 and 20.0 μM, respectively. Besides, the results of antiallergic activities of compounds 6–8 exhibited inhibitory activities with IC₅₀ values of 10.88 ± 3.05, 38.88 ± 3.29, and 2.44 ± 0.17 μg/mL, respectively (IC₅₀ 91.6 μM for the positive control loratadine).

Graphical Abstract

中文翻译：

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来自北极诺卡氏菌 SCSIO 502F 的细胞毒性吩嗪和抗过敏吩恶嗪生物碱

非常适应北冰洋的微生物有望生产新型化合物，因为它们具有奇特的适应策略并且尚未得到充分研究。从Nocardiopsis dassonvillei 502F（一种最初从北极深海沉积物中分离出来的菌株）中分离出两种新的吩嗪生物碱（1和2）和一种新的吩恶嗪（3 ）。对Nocardiopsis dassonvillei 502F基因组的 AntiSMASH 分析揭示了 16 个假定的生物合成基因簇 (BGC) 的存在，其中包括吩嗪 BGC。对大多数分离的化合物的抗菌、抗过敏和细胞毒活性进行了评估。其中，化合物4和5对骨肉瘤细胞系143B表现出有效的体外细胞毒活性，IC ₅₀值分别为0.16和20.0 μM。此外，化合物6 ~ 8的抗过敏活性结果显示出抑制活性，IC ₅₀值分别为10.88±3.05、38.88±3.29和2.44±0.17μg/mL（阳性对照氯雷他定的IC ₅₀ 91.6μM）。

图形概要