The marine environment is a rich reservoir of diverse biological entities, many of which possess unique properties that are of immense value to biotechnological applications. One such example is the red fluorescent protein derived from the coral Discosoma sp. This protein, encoded by the DsRed gene, has been the subject of extensive research due to its potential applications in various fields. In the study, a variant of the red fluorescent protein was generated through random mutagenesis using the DsRed2 gene as a template. The process employed error-prone PCR (epPCR) to introduce random mutations, leading to the isolation of twelve gene variants. Among these, one variant stood out due to its unique spectral properties, exhibiting dual fluorescence emission at both 480 nm (green) and 550 nm (red). This novel variant was expressed in both Escherichia coli and zebrafish (Danio rerio) muscle, confirming the dual fluorescence emission in both model systems. One of the immediate applications of this novel protein variant is in ornamental aquaculture. The dual fluorescence can serve as a unique marker or trait, enhancing the aesthetic appeal of aquatic species in ornamental settings.

海洋环境是多种生物实体的丰富库，其中许多生物实体具有对生物技术应用具有巨大价值的独特特性。其中一个例子是源自珊瑚Discosoma sp 的红色荧光蛋白。这种由 DsRed 基因编码的蛋白质由于其在各个领域的潜在应用而成为广泛研究的主题。在这项研究中，使用 DsRed2 基因作为模板，通过随机诱变生成了红色荧光蛋白的变体。该过程采用易错 PCR (epPCR) 引入随机突变，从而分离出 12 种基因变异。其中，一种变体因其独特的光谱特性而脱颖而出，在 480 nm（绿色）和 550 nm（红色）处表现出双荧光发射。这种新的变体在大肠杆菌和斑马鱼（Danio rerio ）肌肉中都有表达，证实了两个模型系统中的双荧光发射。这种新型蛋白质变体的直接应用之一是观赏水产养殖。双荧光可以作为独特的标记或特征，增强观赏环境中水生物种的审美吸引力。