The transcriptome data of skin cells from domestic cats with brown, orange, and white coats were analyzed using a public database to investigate the possible relationship between coat color-related gene expression and squamous cell carcinoma risk, as well as the mechanism of deafness in white cats. We found that the ratio of the expression level of genes suppressing squamous cell carcinoma to that of genes promoting squamous cell carcinoma might be considerably lower than the theoretical estimation in skin cells with orange and white coats in white-spotted cat. We also found the possibility of the frequent production of KIT lacking the first exon (d1KIT) in skin cells with white coats, and d1KIT production exhibited a substantial negative correlation with the expression of SOX10, which is essential for melanocyte formation and adjustment of hearing function. Additionally, the production of d1KIT was expected to be due to the insulating activity of the feline endogenous retrovirus 1 (FERV1) LTR in the first intron of KIT by its CTCF binding sequence repeat. These results contribute to basic veterinary research to understand the relationship between cat skin coat and disease risk, as well as the underlying mechanism.

中文翻译：

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通过RNA-seq数据推断家猫皮毛颜色依赖性风险的可能性以及鳞状细胞癌和耳聋的危险因素

利用公共数据库对棕色、橙色和白色皮毛家猫的皮肤细胞转录组数据进行分析，探讨毛色相关基因表达与鳞状细胞癌风险之间的可能关系，以及白色耳聋的机制猫。我们发现，在白斑猫的橙色和白色皮毛皮肤细胞中，抑制鳞状细胞癌的基因与促进鳞状细胞癌的基因的表达水平的比率可能大大低于理论估计。我们还发现白衣皮肤细胞中可能频繁产生缺乏第一个外显子（d1KIT）的KIT，并且d1KIT的产生与SOX10的表达呈显着的负相关，而SOX10对于黑色素细胞的形成和听力功能的调节至关重要。此外，d1KIT 的产生预计是由于猫内源性逆转录病毒 1 (FERV1) LTR 在KIT第一个内含子中通过其 CTCF 结合序列重复而具有绝缘活性。这些结果有助于基础兽医研究，以了解猫皮毛与疾病风险之间的关系以及潜在机制。