Micro/nanofluidic devices and systems have gained increasing interest in healthcare applications over the last few decades because of their low cost and ease of customization, with only a small volume of sample fluid required. Many biological queries are now being addressed using various types of single-molecule research. With this rapid rise, the disadvantages of these methods are also becoming obvious. Micro/nanofluidics-based biochemical analysis outperforms traditional approaches in terms of sample volume, turnaround time, ease of operation, and processing efficiency. A complex and multifunctional micro/nanofluidic platform may be used for single-cell manipulation, treatment, detection, and sequencing. We present an overview of the current advances in micro/nanofluidic technology for single-cell research, focusing on cell capture, treatment, and biochemical analyses. The promise of single-cell analysis using micro/ nanofluidics is also highlighted.

中文翻译：

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用于细胞分离技术的纳米/微流体装置的综述：最新进展和进展

过去几十年来，微/纳流体设备和系统在医疗保健应用中引起了越来越多的兴趣，因为它们成本低廉且易于定制，仅需要少量的样品流体。现在正在使用各种类型的单分子研究来解决许多生物学问题。随着这种快速崛起，这些方法的缺点也变得越来越明显。基于微/纳流体的生化分析在样本量、周转时间、操作简便性和处理效率方面优于传统方法。复杂且多功能的微/纳流体平台可用于单细胞操作、处理、检测和测序。我们概述了单细胞研究微/纳流体技术的当前进展，重点是细胞捕获、处理和生化分析。还强调了使用微/纳米流体进行单细胞分析的前景。