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Introgression of 1RSWR.1BL translocation, Bx7OE and stripe rust resistance genes in bread wheat cultivars
Cereal Research Communications ( IF 1.6 ) Pub Date : 2023-10-23 , DOI: 10.1007/s42976-023-00444-9
Sheenu Garg , Ambika Sharma , Satinder Kaur , H. S. Dhaliwal , Pritesh Vyas , Imran Sheikh

The composition of seed storage proteins determines the suitability of bread wheat cultivar for different end-use products. high-molecular-weight glutenin subunits are the major determinants of the processing quality of bread wheat flour. The spontaneous translocation of the 1RS arm of Secale cereale L. in bread wheat is associated with higher grain yield and abiotic stress tolerance, but it leads to deterioration in the end-use quality due to the presence of rye storage secalin protein encoded by Sec-1 locus. The 1RSWR.1BL recombinant line W1635 without Sec-1, over-expressed Glu1-Bx7 (Bx7OE) and two stripe rust resistance genes Yr5 and Yr17 were used as the donor for marker-assisted breeding in the bread wheat cultivars UP2338 and HS240 with 1RS.1BL translocation. A strong positive correlation was found between the micro-SDS sedimentation test (MST) values and Bx7OE in the derivatives along with Glu-D1 (5 + 10) indicating the efficacy of Bx7OE in improving the end-use quality. In addition to this, the introgression of Yr5 and Yr17 genes led to stripe rust resistance in the derivatives for their stable and environment friendly production.



中文翻译:

1RSWR.1BL易位、Bx7OE和条锈病抗性基因在面包小麦品种中的渗入

种子储藏蛋白的组成决定了面包小麦品种对不同最终用途产品的适用性。高分子量麦谷蛋白亚基是面包小麦粉加工质量的主要决定因素。面包小麦中黑麦1RS 臂的自发易位与较高的谷物产量和非生物胁迫耐受性相关,但由于存在由黑麦编码的黑麦储存黑麦素蛋白,导致最终使用质量恶化。 1 个位点。以不含Sec-1、过表达Glu1- Bx7 ( Bx7 OE ) 和两个条锈病抗性基因Yr5Yr17的1RS WR .1BL 重组系 W1635作为供体,在面包小麦品种 UP2338 和 HS240 中进行标记辅助育种具有 1RS.1BL 易位。衍生物中的微 SDS 沉降测试 (MST) 值与Bx7 OE以及Glu-D1 (5 + 10) 之间存在很强的正相关性,表明Bx7 OE在提高最终使用质量方面的功效。除此之外,Yr5Yr17基因的渗入导致衍生物具有抗条锈病性,从而实现稳定且环境友好的生产。

更新日期:2023-10-24
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