This study aimed to determine the prevalence and phylogenetic analysis of Ehrlichia spp. in horses and dogs in Iran. Blood samples were collected from 400 animals, including 200 horses and 200 dogs, from five different provinces in Iran. Polymerase chain reaction (PCR) was used to detect Ehrlichia spp. based on amplification of the 16S rRNA gene. The semi-nested PCR method was used to amplify the dsb, TRP36, and gltA genes. The results showed that 4.5 % of the samples (3 % horses and 6 % dogs) were positive for Ehrlichia sp. The highest prevalence was observed in Kerman and Khuzestan, while the lowest was found in West Azerbaijan, Golestan, and Mazandaran. The study suggests that the populations of dogs and horses in the country should be considered important factors in the epidemiology of ehrlichiosis. Phylogenetic analysis based on the dsb and TRP36 genes revealed that the prevalent species were E. canis and E. ruminantium.

本研究旨在确定埃利希体属的流行率和系统发育分析。伊朗的马和狗。从伊朗五个不同省份的 400 只动物身上采集了血液样本，其中包括 200 匹马和 200 只狗。聚合酶链反应（PCR）用于检测埃里希体属。基于16S rRNA基因的扩增。采用半巢式PCR方法扩增dsb、TRP36和gltA基因。结果显示，4.5% 的样本（3% 马和 6% 狗）埃利希体呈阳性。克尔曼和胡齐斯坦的患病率最高，而西阿塞拜疆、古勒斯坦和马赞德兰的患病率最低。该研究表明，该国的狗和马的数量应被视为埃利希体病流行病学的重要因素。基于dsb和TRP36基因的系统发育分析表明，普遍存在的物种是E. canis和E. ruminantium。