One of the most common vegetable crops in the world is the tomato (Solanum lycopersicum L). Despite an increase in the area under tomato cultivation, production has not kept up. One of the most significant obstacles to the yield of tomato crops is the Alternaria pathogen. In this study, 11 Alternaria species were isolated from tomato leaves and identified using a morphological and molecular tool based on ITS regions of the rDNA sequences. Fe (iron) nanoparticles (NPs) were created in the current investigation utilizing cow urine. The surface morphology and synthesis of metal oxide functional groups were studied using Scanner electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Preliminary x-ray diffraction (XRD) reveals that Fe nanoparticles have a hexagonal structure with a space group (P63mc) (FTIR). Cow urine, Fe nanoparticles, and their combination (CU+ FeNPs) were tested for their effectiveness in vitro and in vivo against the leaf spot pathogen The lethal action of FeNPs on leaf spot may be mediated by ROS, which plays a function in NPs by inhibiting A. alternata growth. In the future, the use of CU + FeNPs combination in crop production mainly to increased yield quality, and manage diseases should be promoted. As far as we know, this is the first attempt made to manage this important pathogen (leaf spot pathogen) in tomato plants using a combination of CU + FeNPs together under field conditions.

世界上最常见的蔬菜作物之一是番茄（Solanum lycopersicum L）。尽管番茄种植面积有所增加，但产量却没有跟上。番茄作物产量最重要的障碍之一是链格孢属病原体。在这项研究中，从番茄叶子中分离出11 种链格孢属物种，并使用基于 rDNA 序列的 ITS 区域的形态学和分子工具进行了鉴定。目前的研究利用牛尿制造了铁（铁）纳米粒子（NP）。使用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）研究了金属氧化物官能团的表面形貌和合成。初步 X 射线衍射 (XRD) 显示 Fe 纳米粒子具有空间群 (P63mc) 的六方结构（FTIR）。测试了牛尿、Fe 纳米粒子及其组合（CU+ FeNPs）在体外和体内对抗叶斑病病原体的有效性 FeNPs 对叶斑病的致死作用可能是由 ROS 介导的，ROS 通过抑制链格孢生长。今后在作物生产中推广CU+FeNPs组合主要是为了提高产量品质、防治病害。据我们所知，这是在田间条件下使用 CU + FeNP 组合来控制番茄植株中这种重要病原体（叶斑病病原体）的首次尝试。