To develop a more environmentally friendly, low-cost, energy-efficient, and non-toxic strategy for producing silver nanoparticles (AgNPs) for cancer therapy, in this study, we established a green synthesis method using Olea europaea fruit extract (OFE) as a reducing and stabilizing agent for the AgNPs. The AgNPs were then characterized using relevant spectroscopy and other analyses, and their cytotoxicity on two breast cancer cells, MCF7 and T47D, were evaluated. The AgNPs exhibited considerable anticancer potential against both MCF7 and T47D cells, inhibiting their proliferation more efficiently than the OFE. The AgNPs-OFE was highly cytotoxic against the T47D cancer cell line with a 50 % inhibitory concentration (IC₅₀) of 77 µg/mL. This study demonstrates the feasibility of using OFE in green synthesizing stable AgNPs with an acceptable particle size and shape and other desirable characteristics, including anticancer activities against the selected in vitro breast cancer cell model. The findings demonstrate the encouraging biomedical and therapeutic application of the Plant-derived AgNPs, which has clinical and academic relevance and warrants further investigation.

为了开发一种更环保、低成本、节能、无毒的策略来生产用于癌症治疗的纳米银（AgNPs），在本研究中，我们建立了一种以油橄榄果实提取物（OFE）为原料的绿色合成方法。 AgNP 的还原剂和稳定剂。然后使用相关光谱和其他分析对 AgNP 进行表征，并评估它们对两种乳腺癌细胞 MCF7 和 T47D 的细胞毒性。AgNPs 对 MCF7 和 T47D 细胞表现出相当大的抗癌潜力，比 OFE 更有效地抑制它们的增殖。AgNPs-OFE 对 T47D 癌细胞系具有高度细胞毒性，50% 抑制浓度 (IC ₅₀ ) 为 77 µg/mL。这项研究证明了使用 OFE 绿色合成稳定的 AgNP 的可行性，该纳米颗粒具有可接受的粒径和形状以及其他所需特性，包括针对选定的体外乳腺癌细胞模型的抗癌活性。研究结果表明，植物源性银纳米粒子具有令人鼓舞的生物医学和治疗应用，具有临床和学术意义，值得进一步研究。