A simple method for producing pseudopregnant mice supports pup production. In this study, pregnant ICR were obtained mice without mating with vasectomized mice via administration of mouse Kisspeptin-10 (mKp-10) and transferring blastocysts to the uterus. Blastocyst transfer after mKp-10 administration to mice with gapping and reddish pink vagina resulted in 65.2% (15/23) pregnancies, and 39.1% (34/87) of the transferred blastocysts showed full-term growth. Vaginal smears were observed for accurate estrus cycle determination, and subsequent administration of mKp10 to mice during the estrus stage and blastocyst transfer resulted in 95.2% (20/21) pregnancies and 50.7% (104/205) birth rates. Regarding 2-cell transfer after administration of mKp-10, 100% (8/8) of the mice became pregnant, and 45.0% (36/80) of the embryos were born. Administration of mKp-10 to mice during the estrus stage is a convenient way to generate pseudopregnant mice.

中文翻译：

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发情期小鼠腹腔注射kisspeptin-10可诱导假孕

一种生产假怀孕小鼠的简单方法支持幼仔的生产。在这项研究中，通过给予小鼠 Kisspeptin-10 (mKp-10) 并将囊胚转移到子宫，获得怀孕的 ICR 小鼠，而无需与输精管切除的小鼠交配。将 mKp-10 给予阴道有裂口和红粉红色的小鼠后，囊胚移植导致 65.2% (15/23) 的妊娠，并且 39.1% (34/87) 的移植囊胚显示足月生长。观察阴道涂片以准确确定发情周期，随后在发情阶段和囊胚移植期间给小鼠施用 mKp10，导致 95.2% (20/21) 的妊娠率和 50.7% (104/205) 的出生率。关于给予mKp-10后的2细胞移植，100%（8/8）的小鼠怀孕，并且45.0%（36/80）的胚胎出生。在发情期给小鼠注射 mKp-10 是产生假孕小鼠的便捷方法。