Abstract

For the first time, an analysis was made of the antioxidant properties of conjugates of CeO₂ nanoparticles with human serum albumin (CeO₂@HSA), including HSA isolated from blood plasma and biological fluids similar in composition to blood plasma, namely, peritoneal (ascitic) and synovial (articular) fluids. The antioxidant activity of hybrid nanomaterials was studied in relation to alkylperoxyl radicals by luminol-dependent chemiluminescence. It was shown that the interaction of CeO₂ nanoparticles with purified human serum albumin is accompanied by a decrease in the antioxidant and prooxidant potential of albumin by a factor of ⁓1.5. Presumably, this is caused by the interaction of nanodispersed CeO₂ with sulfhydryl groups of the protein. Conjugates of CeO₂ nanoparticles with albumin isolated from biological fluids (CeO₂@HSA) exhibit a synergistic antioxidant effect. In this case, the mechanism of antioxidant activity is fundamentally different from that for CeO₂ sols modified with purified human serum albumin. According to quantitative assessment, the antioxidant capacity of CeO₂@HSA conjugates is ⁓20 times lower than that of Trolox, a water-soluble analog of vitamin E.

中文翻译：

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二氧化铈纳米颗粒与生物体液分离人血清白蛋白结合物的抗氧化活性

摘要

首次对CeO ₂纳米粒子与人血清白蛋白（CeO ₂ @HSA）的缀合物的抗氧化特性进行了分析，其中包括从血浆和与血浆成分相似的生物体液（即腹膜）中分离的HSA（腹水）和滑液（关节）液。通过鲁米诺依赖性化学发光研究了杂化纳米材料的抗氧化活性与烷基过氧自由基的关系。结果表明，CeO ₂纳米粒子与纯化的人血清白蛋白的相互作用伴随着白蛋白的抗氧化和促氧化潜力降低1.5倍。_{据推测，这是由纳米分散的CeO 2}与蛋白质的巯基相互作用引起的。CeO ₂纳米粒子与从生物体液中分离的白蛋白（CeO ₂ @HSA）的结合物表现出协同抗氧化作用。_{在这种情况下，抗氧化活性的机制与用纯化人血清白蛋白修饰的CeO 2}溶胶的机制根本不同。根据定量评估，CeO ₂ @HSA缀合物的抗氧化能力比水溶性维生素E类似物Trolox低⁓20倍。