Non-thermal plasma has gained significant popularity in the field of biology due to its ability to generate reactive oxygen and nitrogen species, which can alter gene expression within cells. Plasma treatment has been used to address various medical issues, such as cancer treatment, blood clotting, and bacterial sterilization. In our study, we investigated the effect of non-thermal plasma treatment on monocyte-derived macrophages (U937) in vitro. To create an in vivo-like environment, the cells were embedded in a collagen matrix and treated with a di-electric barrier non-thermal plasma system using a combination of air and helium flow. Our results showed that plasma in air might modulate macrophages through changes in gene expression. Further experiments revealed the presence of reactive species responsible for these changes. By evaluating cell viability and gene expression, we determined that 120 s of plasma treatment in air is optimal for Fibroblast cells within collagen. Our study suggests that non-thermal plasma in air can modulate monocyte-derived macrophages embedded in collagen.

非热等离子体由于能够产生活性氧和氮，从而改变细胞内的基因表达，因此在生物学领域受到广泛欢迎。等离子体治疗已用于解决各种医疗问题，例如癌症治疗、血液凝固和细菌灭菌。在我们的研究中，我们研究了非热等离子体处理对体外单核细胞来源的巨噬细胞 (U937) 的影响。为了创建类似体内的环境，将细胞嵌入胶原基质中，并使用空气和氦流的组合通过介电屏障非热等离子体系统进行处理。我们的结果表明，空气中的血浆可能通过基因表达的变化来调节巨噬细胞。进一步的实验揭示了导致这些变化的活性物质的存在。通过评估细胞活力和基因表达，我们确定空气中 120 秒的等离子体处理对于胶原蛋白中的成纤维细胞来说是最佳的。我们的研究表明，空气中的非热等离子体可以调节嵌入胶原蛋白中的单核细胞衍生的巨噬细胞。