Petal spots are widespread in plants, they are important for attracting pollinators and as economic traits in crop breeding. However, the genetic and developmental control of petal spots has seldom been investigated. To further clarify the development of petal spots formation, we performed comparative transcriptome analysis of Lilium davidii var. unicolor and Lilium davidii petals at the full-bloom stage. In comparison with the parental species L. davidii, petals of the lily variety L. davidii var. unicolor do not have the distinct anthocyanin spots. We show that among 7846 differentially expressed genes detected, LdMYB12 was identified as a candidate gene contributing to spot formation in lily petals. The expression level of LdMYB12 in the petals of L. davidii was higher than that in L. davidii var. unicolor petals. Moreover, overexpression of LdMYB12 led to the appearance of spots on the petals of L. davidii var. unicolor, accompanied by increased expression of anthocyanin synthesis-related genes. Taken together, these results indicate that abnormal expression of LdMYB12 contributes to petal spot deficiency in L. davidii var. unicolor.

花瓣斑点在植物中广泛存在，它们对于吸引传粉媒介和作物育种中的经济性状非常重要。然而，花瓣斑点的遗传和发育控制却很少被研究。为了进一步阐明花瓣斑点形成的发展过程，我们对百合进行了比较转录组分析。盛开阶段的单色和百合花瓣。与亲本品种L. davidii相比，百合品种L. davidii var的花瓣。单色没有明显的花青素斑点。我们发现，在检测到的 7846 个差异表达基因中，LdMYB12被确定为有助于百合花瓣斑点形成的候选基因。LdMYB12在L. davidii花瓣中的表达量高于L. davidii var. 的表达水平。单色花瓣。此外，LdMYB12的过度表达导致L. davidii var . 的花瓣上出现斑点。单色，伴随着花青素合成相关基因表达的增加。综上所述，这些结果表明LdMYB12的异常表达导致了L. davidii var . 的花瓣斑点缺陷。单色。