Salt stress is a major factor restricting the growth, distribution, and yield of crops. Previous studies on NRT (nitrate transporter) have mainly focused on the nitrogen transport and response to metal ion stress. Suaeda salsa L. is a characteristic halophyte that can grow normally at a salt concentration of 200 mM. In this study, we want to know the S. salsa’s NRT-related genes and whether they can improve plant salt stress. So we use bioinformatics and molecular biology to verify our hypothesis. Transcriptome data revealed that the S. salsa NRT1.1D (SsNRT1.1D) gene was upregulated under salt stress. The SsNRT1.1D open reading frame is 1743 bp and has MFS and PTR2 conserved domains. Subcellular localization revealed that SsNRT1.1D is primarily located in the endoplasmic reticulum. qRT-PCR results showed that SsNRT1.1D gene is mainly expressed in S. salsa leaves. The expression of the SsNRT1.1D gene in S. salsa leaves was highest at 12 h after 400 mM sodium chloride (NaCl) treatment. Transgenic tomato plants containing the overexpression vector (35S::SsNRT1.1D) were generated and grown under field conditions to obtain the T₃ generation transgenic tomato seeds. The results showed that transgenic tomato plants had higher salt tolerance than the wild-type plants. Overall, our results demonstrate that the SsNRT1.1D gene can improve plant salt tolerance. This study provides a theoretical basis for improving the utilization of saline-alkaline land and the yield of tomatoes.

盐胁迫是限制农作物生长、分布和产量的主要因素。以往对NRT（硝酸盐转运蛋白）的研究主要集中在氮转运和对金属离子胁迫的响应。碱蓬是一种典型的盐生植物，在盐浓度为 200 mM 时仍能正常生长。在本研究中，我们想了解莎莎的NRT相关基因以及它们是否可以改善植物盐胁迫。因此我们利用生物信息学和分子生物学来验证我们的假设。转录组数据显示，S. salsa NRT1.1D ( SsNRT1.1D ) 基因在盐胁迫下上调。SsNRT1.1D开放阅读框为1743 bp，具有MFS和PTR2保守域。亚细胞定位显示 SsNRT1.1D 主要位于内质网中。qRT-PCR结果显示，SsNRT1.1D基因主要在莎莎叶片中表达。400 mM 氯化钠 (NaCl) 处理后 12 小时，SsNRT1.1D基因在S. salsa叶片中的表达量最高。产生含有过表达载体(35S::SsNRT1.1D)的转基因番茄植物并在田间条件下生长以获得T ₃代转基因番茄种子。结果表明，转基因番茄植株比野生型番茄植株具有更高的耐盐性。总的来说，我们的结果表明SsNRT1.1D基因可以提高植物的耐盐性。该研究为提高盐碱地利用率和番茄产量提供理论依据。