Objective: Kinesin family proteins (KIFs) play crucial roles in human tumorigenesis and progression. This study aimed to investigate the expression and association of Kinesin family member 20B (KIF20B) with lung adenocarcinoma (LUAD).
Methods: RNA-seq data from LUAD patients (n = 535) were extracted from TCGA. KIF20B expression was compared between tumor tissues and controls, and between different stages of the disease. Survival and Cox regression analyses were performed, as well as in vitro cellular experiments on A549 cells.
Results: KIF20B is upregulated in LUAD tumor tissues compared with controls and is higher in advanced stages. Patients with high expression of KIF20B have shorter survival times. KIF20B is an independent risk factor for the prognosis of LUAD. High KIF20B expression samples were enriched in signaling pathways related to tumor progression. si-KIF20B transfection reduced migration and invasion of A549 cells and increased apoptosis. The expression of p53 and Bax proteins was upregulated by si-KIF20B, while Bcl-2 was down-regulated.
Discussion: This study reveals that high KIF20B expression is an independent risk factor for the poor prognosis of LUAD. The inhibition of KIF20B might be of great value for suppressing LUAD progression.

中文翻译：

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KIF20B 与 LUAD 进展相关并且是一个独立的危险因素

目的：驱动蛋白家族蛋白（KIF）在人类肿瘤的发生和进展中发挥着至关重要的作用。本研究旨在探讨驱动蛋白家族成员 20B (KIF20B) 的表达及其与肺腺癌 (LUAD) 的关联。
方法：从 TCGA 中提取 LUAD 患者 (n = 535) 的 RNA-seq 数据。比较肿瘤组织和对照之间以及疾病不同阶段之间的 KIF20B 表达。对 A549 细胞进行了生存和 Cox 回归分析以及体外细胞实验。
结果：与对照相比，KIF20B 在 LUAD 肿瘤组织中表达上调，并且在晚期更高。KIF20B高表达的患者生存时间较短。KIF20B是LUAD预后的独立危险因素。高 KIF20B 表达样本富含与肿瘤进展相关的信号通路。si-KIF20B 转染减少了 A549 细胞的迁移和侵袭并增加了细胞凋亡。si-KIF20B 上调 p53 和 Bax 蛋白的表达，而 Bcl-2 蛋白的表达下调。
讨论：本研究揭示KIF20B高表达是LUAD预后不良的独立危险因素。抑制 KIF20B 可能对于抑制 LUAD 进展具有重要价值。