Obesity is a health condition accompanied by life-threatening comorbidities; hence, there is an increasing need for anti-obesity agents. The anti-cancer effects of the leaves of Asimina triloba (pawpaw) has been reported. However, limited research has been conducted on the potential anti-obesity effects of A. triloba fruit. Therefore, this study aimed to explore the effects of A. triloba fruit extract on murine preadipocytes (3T3-L1). We specifically examined lipid droplet formation in these cells using Oil Red O solution and intracellular pro-adipogenic protein levels were examined using western blot analysis. The results revealed that treatment with A. triloba 70% ethanolic fruit extract effectively suppressed lipid droplet formation. Moreover, the expression of crucial proteins involved in adipogenesis, namely sterol regulatory element-binding protein 1, peroxisome proliferator-activated receptor γ, and fatty acid synthase, were significantly inhibited. These findings suggest that A. triloba fruit has the potential to prevent obesity by inhibiting fat synthesis and may serve as a natural source for anti-obesity functional agents. .

中文翻译：

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Asimina triloba（木瓜）果实提取物抑制 3T3-L1 细胞中的脂肪细胞分化和脂肪生成相关蛋白表达

肥胖是一种健康状况，伴有危及生命的合并症；因此，对抗肥胖剂的需求不断增加。据报道，木瓜叶具有抗癌作用。然而，关于三叶草果实潜在的抗肥胖作用的研究有限。因此，本研究旨在探讨三叶草果实提取物对小鼠前脂肪细胞（3T3-L1）的影响。我们使用油红 O 溶液专门检查了这些细胞中脂滴的形成，并使用蛋白质印迹分析检查了细胞内促脂肪形成蛋白水平。结果表明，用三叶草 70% 乙醇果实提取物处理可有效抑制脂滴形成。此外，参与脂肪生成的关键蛋白，即甾醇调节元件结合蛋白1、过氧化物酶体增殖物激活受体γ和脂肪酸合酶的表达被显着抑制。这些发现表明，三叶草果实具有通过抑制脂肪合成来预防肥胖的潜力，并且可以作为抗肥胖功能剂的天然来源。。