The objective of this study is to further investigate the feasibility of Zinc–Titanium implant as a potential implantable material in oral application in aspects of osteoblast biocompatibility, osteogenesis and osseointegration ability. First, we used plasma immersion ion implantation and deposition (PIIID) technology to introduce Zinc ion into pure Titanium surface, then we used X-ray photoelectron spectroscopy to analyze the chemical composition of modified surface layer; next, we used in vitro studies including immunological fluorescence assay and western blotting to determine responses between MG-63 osteoblast-like cell and implant. In vivo studies adopted pig model to check the feasibility of Zn–Ti implant. Results showed that in vitro and in vivo were consistent, showing that Zn ion was successfully introduced into Ti surface by PIIID technique. The chemical and physical change on modified plant resulted in the more active expressions of mRNA and protein of Type I collagen in MG-63 cells compared with non-treated implant, and the better integration ability of bones with modified implant. We confirmed the Zn–Ti implant owns the ability in promoting osteogenesis and osteointegration in early phase of implantation and is a qualified candidate in dentistry. The overview of our study can be depicted as follows.

Graphical Abstract

中文翻译：

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将锌离子掺入钛表面促进植入早期的成骨和骨整合

本研究的目的是进一步探讨锌钛种植体作为潜在的口腔植入材料在成骨细胞生物相容性、成骨和骨整合能力方面的可行性。首先，我们利用等离子体浸没离子注入和沉积（PIIID）技术将锌离子引入纯钛表面，然后利用X射线光电子能谱分析改性表面层的化学成分；接下来，我们使用包括免疫荧光测定和蛋白质印迹在内的体外研究来确定 MG-63 成骨细胞样细胞和植入物之间的反应。体内研究采用猪模型来检验锌钛植入物的可行性。结果表明，体外和体内结果一致，表明PIIID技术成功地将Zn离子引入到Ti表面。改性植物的化学和物理变化导致MG-63细胞中I型胶原mRNA和蛋白的表达比未处理的植入物更加活跃，并且改性植入物的骨骼整合能力更好。我们确认Zn-Ti种植体在种植早期具有促进成骨和骨整合的能力，是牙科领域的合格候选者。我们的研究概述如下。

图形概要