High-speed atomic force microscopy is now a widely used technique to study dynamics of single biomolecules and complex structures. In the past, it has mainly been used to capture surface topography as structural analysis, leading to important discoveries not attainable by other methods. Similar to conventional atomic force microscopy, the scope of high-speed atomic force microscopy was recently expanded to encompass quantities beyond topography, such as the measurement of mechanical properties. This review delves into various methodologies for assessing mechanical properties, ranging from semi-quantitative approaches to precise force measurements and their corresponding sample responses. We will focus on the application to single proteins such as BIN1, ion channels such as Piezo1, complex structures such as microtubules and, supramolecular fibers. In all these examples, the unique combination of quantifiable force application and high spatiotemporal resolution allows to unravel mechanisms that cannot be investigated by conventional means.

中文翻译：

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使用高速原子力显微镜测量机械性能。

高速原子力显微镜现已成为研究单个生物分子和复杂结构动力学的广泛使用的技术。过去，它主要用于捕获表面形貌作为结构分析，从而获得其他方法无法获得的重要发现。与传统原子力显微镜类似，高速原子力显微镜的范围最近扩展到包括形貌之外的量，例如机械性能的测量。本综述深入研究了评估机械性能的各种方法，从半定量方法到精确的力测量及其相应的样品响应。我们将重点关注对 BIN1 等单一蛋白质、Piezo1 等离子通道、微管和超分子纤维等复杂结构的应用。在所有这些例子中，可量化的力施加和高时空分辨率的独特组合使得能够解开传统手段无法研究的机制。