As an important food and cash crop, identification of DNA molecular markers is of great significance for molecular marker-assisted breeding of Sorghum (Sorghum bicolor (L.) moench). Although some sorghum-related mutation databases have been published, the special SSR and SV databases still need to be constructed and updated. In this study, the quality of 18 different sorghum genomes was evaluated, and two genomes were assembled at chromosome level. Through the identification and comparative analysis of SSR loci in these genomes, the distribution characteristics of SSR in the above sorghum genomes were initially revealed. At the same time, five representative reference genomes were selected to identify the structural variation of sorghum. Finally, a convenient SSR/SV database of sorghum was constructed by integrating the above results ( http://www.sorghum.top:8079/ ; http://43.154.129.150:8079/ ; http://47.106.184.91:8079/ ). Users can query the information of related sites and primer pairs. Anyway, our research provides convenience for sorghum researchers and will play an active role in sorghum molecular marker-assisted breeding.

中文翻译：

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SSR/SV多基因组综合鉴定及分子标记数据库开发服务高粱育种

作为重要的粮食和经济作物，DNA分子标记的鉴定对于高粱(Sorghum bicolor (L.) moench)的分子标记辅助育种具有重要意义。尽管一些高粱相关突变数据库已经发表，但特殊的SSR和SV数据库仍需要构建和更新。本研究评估了18个不同高粱基因组的质量，并在染色体水平上组装了两个基因组。通过对这些基因组中SSR位点的鉴定和比较分析，初步揭示了SSR在上述高粱基因组中的分布特征。同时，选取了5个有代表性的参考基因组来鉴定高粱的结构变异。最后，综合上述结果构建了一个方便的高粱SSR/SV数据库（http://www.sorghum.top:8079/；http://43.154.129.150:8079/；http://47.106.184.91： 8079/）。用户可以查询相关位点和引物对的信息。不管怎样，我们的研究为高粱研究人员提供了便利，将为高粱分子标记辅助育种发挥积极作用。