Objectives

Plant viruses are a significant contributor to the economic losses experienced in numerous plant species. Begomoviruses exhibit a wide range of host species, encompassing both cultivated and non-cultivated plants. The kudzu plant, scientifically known as Pueraria montana, is indigenous to the Southeast Asian region. Moreover, it exhibits varied attributes with regards to its utilization in the fields of medicine, food, cosmetics, and the herbal industry.

Methods

During a routine survey, some P. montana plants having typical symptoms of yellowing, mosaic, and mottle were observed in areas including Fort-Minro (FM) and Anari of district Dera Ghazi Khan and Maari (MA) of district Rajanpur. The genomic DNA was extracted from the collected samples of these areas. The polymerase chain reaction (PCR) and rolling circular amplification (RCA) was carried out to isolate the DNA and DNA- β from the collected plant specimens. An evolutionary research was conducted to assess the lineage of this virus inside a novel geographical region.

Results

A 500-bp amplicon size was obtained from the samples of the FM and MA, while the approximately 2.7-kb amplicon size of its full genome was obtained from the samples of both locations. The results of the sequence analysis demonstrated conclusively that the plants in question are infected with Kudzu mosaic virus (KMV). The evolutionary history analysis has confirmed the virus's lineage in a new region, and the nucleotide frequencies of obtained sequences were variable.

Conclusion

The presence of a novel bipartite begomovirus in the tropical region has been substantiated using a phylogenetic and evolutionary investigation. The observation of a white fly population on infected plants has also suggested that the transmission of this virus through the migration of this insect vector can be a viable mechanism.

中文翻译：

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感染葛根（Pueraria montana）的新型葛花叶病毒（KMV）的分子特征和进化历史分析

目标

植物病毒是造成许多植物物种经济损失的重要因素。贝戈莫病毒表现出广泛的宿主物种，包括栽培植物和非栽培植物。葛植物，科学上称为蒙大拿葛根，原产于东南亚地区。此外，它在医药、食品、化妆品和草药工业领域的应用方面表现出不同的属性。

方法

在例行调查中，在德拉加齐汗区的 Fort-Minro (FM) 和 Anari 以及 Rajanpur 区的 Maari (MA) 等地区观察到一些蒙大拿植物具有黄化、花叶和斑驳的典型症状。从这些地区收集的样本中提取了基因组 DNA。进行聚合酶链反应（PCR）和滚环扩增（RCA）从收集的植物标本中分离DNA和DNA-β。进行了一项进化研究，以评估该病毒在新地理区域内的谱系。

结果

从 FM 和 MA 的样本中获得了 500 bp 的扩增子大小，而从两个位置的样本中获得了其全基因组的大约 2.7 kb 的扩增子大小。序列分析的结果最终证明，所讨论的植物感染了葛花叶病毒（KMV）。进化历史分析证实了该病毒在新区域的谱系，并且获得的序列的核苷酸频率是可变的。

结论

系统发育和进化研究证实了热带地区存在一种新型二分贝戈莫病毒。对受感染植物上粉虱种群的观察也表明，通过这种昆虫媒介的迁移传播这种病毒可能是一种可行的机制。