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Evaluation and chemical profiling of different Centaurea iberica extracts and investigation of different in vitro biological activities
Journal of King Saud University-Science ( IF 3.8 ) Pub Date : 2023-11-04 , DOI: 10.1016/j.jksus.2023.102992
Haleema Bibi , Javed Iqbal , Banzeer Ahsan Abbasi , Sobia Kanwal , Maryam Tavafoghi , Mohammad Z. Ahmed , Tariq Mahmood

In almost all cultures medicinal plants are used as a rich source of phytochemicals and nutraceuticals components and have played promising role in maintaining and improving human health. The current study was aimed to unveil biological evaluation of Centaurea iberica. Plant extracts were prepared in five different solvents viz. methanol, ethanol, n-hexane, ethyl acetate and chloroform. The antioxidant potentials (2,2-diphenyl, 1-picrylhydrazine scavenging activity, total reducing power) of the plant revealed that the methanolic extract showed significant DPPH (2,2-diphenyl, 1-picrylhydrazine) scavenging activity (IC50 value 77.20 ± 8.81), reducing power (20.45 ± 0.54 AAE mg/g). In case of antibacterial assay, highest zone of inhibition (20 ± 1.5 mm) was recorded in methanolic extract against Klebsiella pneumonia. Likewise, for antifungal activity the highest zone of inhibition was recorded for methanolic extract (18 ± 2.4 mm) against Aspergillus niger. Moreover, 50 % inhibition was observed for methanolic extract against targated PC3 (prostate cancer cell line) cell lines and 3T3 cell lines. In the light of present study, it is recommended that further in vitro and in vivo studies should be performed using different animal models and characterization should be done to isolate different compounds that could be used to treat different types of ailments.



中文翻译:

不同矢车菊提取物的评价和化学分析以及不同体外生物活性的研究

在几乎所有文化中,药用植物都被用作植物化学物质和营养保健成分的丰富来源,并在维持和改善人类健康方面发挥了有前景的作用。目前的研究旨在揭示矢车菊的生物学评价。植物提取物在五种不同的溶剂中制备,即。甲醇、乙醇、正己烷、乙酸乙酯和氯仿。植物的抗氧化潜力(2,2-二苯基,1-三硝基肼清除活性,总还原力)表明,甲醇提取物表现出显着的DPPH(2,2-二苯基,1-三硝基肼)清除活性(IC 50 值77.20 ± 8.81),还原能力(20.45 ± 0.54 AAE mg/g)。在抗菌测定中,甲醇提取物对肺炎克雷伯菌记录了最高抑制区(20 ± 1.5 mm) 。同样,对于抗真菌活性,记录了甲醇提取物对黑曲霉的最高抑制区(18 ± 2.4 mm 。此外,观察到甲醇提取物对靶向 PC3(前列腺癌细胞系)细胞系和 3T3 细胞系有 50% 的抑制作用。根据目前的研究,建议使用不同的动物模型进行进一步的体外体内研究,并进行表征以分离可用于治疗不同类型疾病的不同化合物。

更新日期:2023-11-04
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