The adaptive and innate immune system is important in both initiating and preventing functional disorders during pregnancy, one of which is pre-eclampsia. The research aims to conduct the comparative quantification of selected subpopulations of peripheral blood immunoregulatory cells in pregnant women with pre-eclampsia in the third trimester. The marker receptors CD4, CD8, CD95, CD25, CD27, and the marker antigen HLA-DR were considered. The screening was performed by flow cytometry with dual phenotyping using phycoerythrin- and fluorescein-isothiocyanate-labeled monoclonal antibodies. Data processing consisted in calculating a likelihood value to assess the statistical significance of the difference between the samples. A statistically significant decrease in the subpopulation titer of T- and B-lymphocytes with marker receptors CD4, CD8, and CD19 was found in pre-eclampsia patients. In the CD4 carrier T-lymphocyte population, there was an increased expression of the CD25/CD95 activation and apoptosis markers. In the CD8 T-killer population, a decreased representation of the CD27/CD25/CD95 markers of differentiation, activation, and apoptosis was deterministic. The expression pattern of the major histocompatibility complex antigen HLA-DR did not change significantly in normality and pathology. The titer of peripheral natural killer cells carrying the CD56 marker increased in patients with various degrees of disease severity, while the number of CD16 natural killer remained at the level of the control group. The research results suggest that a change in the ratio of the above receptors is a diagnostic indicator for pre-eclampsia.

中文翻译：

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血液淋巴细胞活化标志物对先兆子痫的诊断意义

适应性和先天免疫系统对于孕期功能性疾病的发生和预防都很重要，其中之一就是先兆子痫。该研究旨在对妊娠晚期先兆子痫孕妇的选定外周血免疫调节细胞亚群进行比较定量。考虑了标记受体 CD4、CD8、CD95、CD25、CD27 和标记抗原 HLA-DR。使用藻红蛋白和异硫氰酸荧光素标记的单克隆抗体通过流式细胞术进行双表型筛选。数据处理包括计算似然值以评估样本之间差异的统计显着性。在先兆子痫患者中发现带有标记受体 CD4、CD8 和 CD19 的 T 淋巴细胞和 B 淋巴细胞亚群滴度有统计学意义的显着下降。在 CD4 携带 T 淋巴细胞群中，CD25/CD95 激活和凋亡标记物的表达增加。在 CD8 T 杀伤细胞群体中，分化、激活和凋亡的 CD27/CD25/CD95 标记物的减少是确定性的。主要组织相容性复合物抗原HLA-DR的表达模式在正常和病理状态下没有显着变化。不同程度疾病严重程度的患者外周血携带CD56标记物的自然杀伤细胞滴度有所增加，而CD16自然杀伤细胞的数量仍维持在对照组水平。研究结果提示，上述受体比例的变化是先兆子痫的诊断指标。