Background

Carbon monoxide (CO) poisoning affects several vital organs; however, the long-term damage to lungs has not been well investigated.

Objective

This study investigated the morphological changes and the associated molecules 6 weeks after CO poisoning in rat models. Rats in the CO poisoning group were exposed to 1500 ppm CO in a gas chamber for 3 h. After 6 weeks, the rats in the control and CO poisoning groups were sacrificed. Pathological changes were assessed using hematoxylin and eosin staining. Western blotting and antibody arrays for inflammatory signaling molecules were performed using lung homogenates. Complete blood counts and Krebs Von Den Lungen (KL)-6 levels were compared among rats in the control group and those immediately and 6 weeks after CO poisoning using blood sampling from an indwelling catheter.

Results

CO-poisoned lungs showed emphysematous changes with increased mean linear intercept (p = 0.0007). SERPINA1, IL-13, CD44, and GDF7 levels were significantly decreased, whereas TGFβ, ɑ-SMA, MMP9, and NF-κB levels were significantly increased in the CO-poisoned lungs. KL-6 serum levels were significantly higher in the CO-poisoned rats (p < 0.0001).

Conclusion

This is the first study to demonstrate the long-term effects and emphysematous changes in CO-poisoned lung models. We recommend long-term monitoring following CO poisoning.

中文翻译：

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一氧化碳中毒大鼠肺泡损伤及肺气肿发展

背景

一氧化碳 (CO) 中毒会影响多个重要器官；然而，对肺部的长期损害尚未得到充分研究。

客观的

本研究调查了大鼠模型一氧化碳中毒后 6 周的形态变化和相关分子。CO中毒组的大鼠在毒气室中暴露于1500 ppm CO 3小时。6周后处死对照组和CO中毒组大鼠。使用苏木精和伊红染色评估病理变化。使用肺匀浆进行炎症信号分子的蛋白质印迹和抗体阵列。使用留置导管采血，比较对照组大鼠以及 CO 中毒后立即和 6 周大鼠的全血细胞计数和 Krebs Von Den Lungen (KL)-6 水平。

结果

一氧化碳中毒的肺部表现出肺气肿变化，平均线性截距增加（p  = 0.0007）。CO中毒肺中SERPINA1、IL-13、CD44和GDF7水平显着降低，而TGFβ、ɑ-SMA、MMP9和NF-κB水平显着升高。CO 中毒大鼠的 KL-6 血清水平显着升高 ( p  < 0.0001)。

结论

这是第一项证明一氧化碳中毒肺模型的长期影响和肺气肿变化的研究。我们建议对一氧化碳中毒后进行长期监测。