Background

The mucus biomembrane is a primary barrier in delivering drugs to the brain via intranasal delivery. The negatively charged nanoformulations suffer from poor mucoadhesive ability and less retention time in the nasal cavity, which limits further therapeutic efficacy. The positively charged chitosan coating on liposomes may overcome the above issues. Hence, understanding the molecular interactions between the chitosan-coated liposomes and mucin is essential for developing an effective drug delivery system.

Methods

The molecular interactions of mucin with sinapic acid-loaded liposomes (SA-LPs) and mucin with chitosan-coated sinapic acid-loaded liposomes (SA-CH-LPs) were assessed using different biophysical instrumental analyses by interpreting the UV–Vis spectra and observing the particle size, polydispersity index, surface charge, and rheological behavior.

Results

The mucin interaction with SA-CH-LPs showed increased viscosity as compared to SA-LPs with mucin. Moreover, the mucin interaction with SA-CH-LPs showed stronger mucoadhesive properties as compared to SA-LPs with mucin. The electrostatic interaction between positively charged SA-CH-LPs and negatively charged mucin was responsible for the enhanced mucoadhesive property.

Conclusion

The positively charged SA-CH-LPs highly interact with mucin as compared to negatively charged SA-LPs. The mucoadhesive property of SA-CH-LPs could improve the retention of SA in the nasal cavity as compared to SA-LPs. These findings emphasize the importance of chitosan in modulating the mucoadhesive behavior of liposomes.

General significance

Overall, this study helps to understand the molecular interactions and mucoadhesive nature of the chitosan-coated liposomes with mucin, which is essential for biological activity in the physiological environment.

中文翻译：

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壳聚糖包被芥子酸脂质体与粘蛋白分子相互作用的生物物理分析

背景

粘液生物膜是通过鼻内递送将药物递送至大脑的主要屏障。带负电荷的纳米制剂的粘膜粘附能力较差，在鼻腔中的保留时间较短，这限制了进一步的治疗效果。脂质体上带正电荷的壳聚糖涂层可以克服上述问题。因此，了解壳聚糖包被的脂质体和粘蛋白之间的分子相互作用对于开发有效的药物递送系统至关重要。

方法

通过解释紫外-可见光谱和观察，使用不同的生物物理仪器分析评估了粘蛋白与芥子酸负载脂质体（SA-LP）和粘蛋白与壳聚糖包被芥子酸负载脂质体（SA-CH-LP）的分子相互作用。粒度、多分散指数、表面电荷和流变行为。

结果

与SA-LPs与粘蛋白相比，粘蛋白与SA-CH-LPs的相互作用显示出粘度增加。此外，与SA-LPs与粘蛋白相比，粘蛋白与SA-CH-LPs的相互作用显示出更强的粘膜粘附特性​​。带正电荷的 SA-CH-LP 和带负电荷的粘蛋白之间的静电相互作用是增强粘膜粘附性能的原因。

结论

与带负电荷的 SA-LP 相比，带正电荷的 SA-CH-LP 与粘蛋白高度相互作用。与SA-LPs相比，SA-CH-LPs的粘膜粘附特性​​可以改善SA在鼻腔中的保留。这些发现强调了壳聚糖在调节脂质体粘膜粘附行为中的重要性。

一般意义

总的来说，这项研究有助于了解壳聚糖包被的脂质体与粘蛋白的分子相互作用和粘膜粘附性质，这对于生理环境中的生物活性至关重要。