Background: Biosensors and MEMS have witnessed rapid development and enormous interest over the past decades. Constant advancement in diagnostic, medical, and chemical applications has been demonstrated in several platforms and tools. In this study, the analytical and FEA of the microcantilever used in biomolecular analyses were compared with the experimental analysis results. Methods: In this study, MITF antigen, which is a melanoma biomarker, and anti-MITF antibody (D5) were selected as biomolecules. A MEMS-type microcantilever biosensor was designed by functionalizing the AFM cantilever by utilizing the specific interaction dynamics and intermolecular binding ability between both molecules. Surface functionalization of cantilever micro biosensors was performed by using FEA. The stress that will occur as a result of the interactions between the MITF-D5 has been determined from the deviation in the resonant frequency of the cantilever. Results: It has been found that the simulation results are supported by analytical calculations and experimental results. Conclusion: The fact that the results of the simulation study overlap with the experimental and mathematical results allows us to get much cheaper and faster answers compared to expensive and time-consuming experimental approaches.

中文翻译：

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MITF抗原与D5单克隆抗体相互作用的微悬臂生物传感器的设计与模拟有限元分析及实验

背景：过去几十年来，生物传感器和 MEMS 得到了快速发展和巨大的关注。诊断、医疗和化学应用的不断进步已在多个平台和工具中得以体现。本研究将生物分子分析中使用的微悬臂梁的分析和有限元分析与实验分析结果进行了比较。方法：在本研究中，选择黑色素瘤生物标志物 MITF 抗原和抗 MITF 抗体（D5）作为生物分子。利用两个分子之间特定的相互作用动力学和分子间结合能力，通过对 AFM 悬臂进行功能化，设计了 MEMS 型微悬臂生物传感器。使用有限元分析（FEA）对悬臂微型生物传感器进行表面功能化。MITF-D5 之间相互作用所产生的应力已根据悬臂谐振频率的偏差确定。结果：发现模拟结果得到了分析计算和实验结果的支持。结论：与昂贵且耗时的实验方法相比，模拟研究的结果与实验和数学结果重叠，使我们能够更便宜、更快地获得答案。