Background: The species Pterodon emarginatus and P. pubescens, popularly known as white sucupira or faveira, are native to the Cerrado biome and have the potential for medicinal use and reforestation. They are sister species with evolutionary proximity. Objective: Considering that the chloroplast genome exhibits a conserved structure and genes, the analysis of its sequences can contribute to the understanding of evolutionary, phylogenetic, and diversity issues. Methods: The chloroplast genomes of P. emarginatus and P. pubescens were sequenced on the Illumina MiSeq platform. The genomes were assembled based on the de novo strategy. We performed the annotation of the genes and the repetitive regions of the genomes. The nucleotide diversity and phylogenetic relationships were analyzed using the gene sequences of these species and others of the Leguminosae family, whose genomes are available in databases. Results: The complete chloroplast genome of P. emarginatus is 159,877 bp, and that of P. pubescens is 159,873 bp. The genomes of both species have circular and quadripartite structures. A total of 127 genes were predicted in both species, including 110 single-copy genes and 17 duplicated genes in the inverted regions. 141 microsatellite regions were identified in P. emarginatus and 140 in P. pubescens. The nucleotide diversity estimates of the gene regions in twenty-one species of the Leguminosae family were 0.062 in LSC, 0.086 in SSC, and 0.036 in IR. The phylogenetic analysis demonstrated the proximity between the genera Pterodon and Dipteryx, both from the clade Dipterygeae. Ten pairs of primers with potential for the development of molecular markers were designed. Conclusion: The genetic information obtained on the chloroplast genomes of P. emarginatus and P. pubescens presented here reinforces the similarity and evolutionary proximity between these species, with a similarity percentage of 99.8%.

中文翻译：

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Pterodon emarginatus Vogel 和 Pterodon pubescens Benth 的完整叶绿体基因组：比较和系统发育分析

背景：Pterodon emarginatus 和 P. pubescens 物种通常被称为白色 sucupira 或 faveira，原产于塞拉多生物群落，具有药用和重新造林的潜力。它们是进化上接近的姐妹物种。目的：考虑到叶绿体基因组表现出保守的结构和基因，对其序列的分析有助于理解进化、系统发育和多样性问题。方法：在 Illumina MiSeq 平台上对 P. emarginatus 和 P. pubescens 的叶绿体基因组进行测序。基因组是根据从头策略组装的。我们对基因和基因组的重复区域进行了注释。使用这些物种和豆科其他物种的基因序列分析了核苷酸多样性和系统发育关系，这些物种的基因组可在数据库中找到。结果： P. emarginatus 完整叶绿体基因组为 159,877 bp，P. pubescens 完整叶绿体基因组为 159,873 bp。这两个物种的基因组都具有圆形和四部分结构。在这两个物种中总共预测了127个基因，其中包括110个单拷贝基因和17个反向区域的重复基因。在 P. emarginatus 中鉴定出 141 个微卫星区域，在 P. pubescens 中鉴定出 140 个微卫星区域。豆科 21 个物种的基因区域的核苷酸多样性估计值在 LSC 中为 0.062，在 SSC 中为 0.086，在 IR 中为 0.036。系统发育分析表明翼龙属和双翅目属之间的相似性，两者都来自双翅目分支。设计了十对具有开发分子标记潜力的引物。结论：本文提出的 P. emarginatus 和 P. pubescens 叶绿体基因组的遗传信息增强了这些物种之间的相似性和进化接近性，相似性百分比为 99.8%。