Aflatunia ulmifolia (Franch.) Vassilcz. (Rosaceae) is an ornamental plant commonly used for landscaping and is known to form hybrids with other species that have characteristics like frost-resistant and non-rotting rootstocks. However, it is listed as an endangered species in Kazakhstan and is difficult to grow through traditional propagation; thus, in the present study, in vitro regeneration using seed and nodal explants was attempted. Murashige and Skoog (MS), Woody Plant Medium, and Gamborg medium supplemented with different plant growth regulators were utilized. Maximum germination (51.7±4.4%) was achieved by supplementing MS medium with 1.0 mg L^-1 6-benzyladenine (BA) in which maximum shoot length (1.91±0.11 cm), leaves per shoot (8.48±0.32), and shoots per seed (1.83±0.15) were also observed after 12 wk. Comparatively, nodal explants proved better as 58.9±2.9% bud break was noted, inducing 2.84±0.18 shoots per explant with 0.74±0.01 cm length and 5.96±0.17 leaves per shoot at 1.0 mg L^-1 BA after 8 wk. Nodal derived shoots were further multiplied on MS medium in the presence of 1.5 mg L^-1 BA where 3.90±0.32 shoots (96.7% response) formed with 1.33±0.04 cm length after 4 wk. These shoots were rooted in MS media augmented with 0.5 mg L^-1 indole-3-acetic acid, which induced 3.5±0.3 roots per shoot (83.3% response) with 3.8±0.1 cm root length at the end of 4 wk. The plantlets were then transferred to peat:vermiculite (1:1 w/w) for hardening and acclimatization and were finally transferred in the open ground with 69.4% survival rate after 1 mo. This micropropagation protocol will be helpful for the conservation of the genetic resources and biodiversity for A. ulmifolia.

Aflatunia ulmifolia (Franch.) Vassilcz。（蔷薇科）是一种常用于园林绿化的观赏植物，已知可以与具有抗冻和不腐烂砧木等特性的其他物种形成杂交种。但在哈萨克斯坦被列为濒危物种，很难通过传统繁殖方式种植；因此，在本研究中，尝试使用种子和节点外植体进行体外再生。使用补充有不同植物生长调节剂的Murashige和Skoog (MS)、木本植物培养基和Gamborg培养基。通过在 MS 培养基中添加 1.0 mg L ^-1 6-苄基腺嘌呤 (BA) 实现最大发芽率 (51.7±4.4%)，其中最大芽长 (1.91±0.11 cm)、每芽叶数 (8.48±0.32) 和每芽数12周后还观察到种子(1.83±0.15)。相比之下，节点外植体被证明更好，注意到58.9±2.9％的芽破裂，在8周后在1.0mg L -1 BA下，每个外植体诱导2.84±0.18个芽，长度为0.74±0.01cm，每个芽诱导5.96±0.17片叶子^。在存在1.5mg L ^-1 BA的情况下，在MS培养基上进一步繁殖节点衍生的枝条，其中4周后形成3.90±0.32个枝条（96.7%响应），长度为1.33±0.04cm。这些芽在添加了 0.5 mg L ^-1吲哚-3-乙酸的 MS 培养基中生根，在 4 周结束时诱导每个芽 3.5±0.3 个根（83.3% 响应），根长为 3.8±0.1 cm。然后将幼苗转移到泥炭：蛭石（1：1 w/w）中进行硬化和适应，最后转移到露天，1个月后成活率为69.4％。该微繁殖方案将有助于保护A. ulmifolia的遗传资源和生物多样性。