Background

Interleukin (IL)-41 is a recently discovered secreted protein that is expressed in a variety of tissues, and it is associated with several immune and metabolic diseases. However, IL-41 has not been studied in hyperuricemia (HUA).

Methods

Forty-four HUA patients and 44 healthy controls (HCs) were included in this study, and we collected theirgeneral and biochemical parameters, including white blood cell, neutrophil, lymphocyte, and platelet counts, mean platelet volume, platelet distribution width, serum creatinine, blood urea nitrogen, fasting blood glucose, total triglyceride, total cholesterol, high-density lipoprotein, low-density lipoprotein, total protein, albumin, alkaline phosphatase, gamma-glutamyltransferase, and hemoglobin concentration. The level of serum IL-41 was determined using an enzyme-linked immunosorbent assay. Multivariate logistic regression analysis was exploited to identify the independent risk factors associated with HUA, and the clinical diagnostic value of IL-41 was analyzed by applying the receiver operating characteristic (ROC) curve. We assessed the association between IL-41 and clinical parameters with Spearman's rank correlation.

Results

Circulating IL-41 levels were significantly higher in HUA patients than in the HCs group (460.3 pg/mL vs. 261.3 pg/mL, respectively; P < 0.001). The area under the ROC curve (AUC) for IL-41 in HUA patients was 0.7443 (with a cut-off value of 311.055 pg/mL, a sensitivity of 68.18 %, and a specificity of 72.73 %), while the AUC for IL-41 combined with the platelet count was 0.8109. Correlation analysis revealed that the circulating IL-41 level was positively correlated with age in HCs and HUA patients.

Conclusions

We herein demonstrated that serum IL-41 was elevated in HUA patients and that it may constitute a novel biomarker of anti-inflammatory response related to HUA.

中文翻译：

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Interleukin-41 作为与高尿酸血症相关的抗炎反应的生物标志物

背景

白细胞介素(IL)-41是最近发现的一种分泌蛋白，在多种组织中表达，与多种免疫和代谢疾病相关。然而，IL-41 尚未在高尿酸血症 (HUA) 中进行研究。

方法

本研究纳入了 44 名 HUA 患者和 44 名健康对照（HC），我们收集了他们的一般和生化参数，包括白细胞、中性粒细胞、淋巴细胞和血小板计数、平均血小板体积、血小板分布宽度、血清肌酐、血尿素氮、空腹血糖、总甘油三酯、总胆固醇、高密度脂蛋白、低密度脂蛋白、总蛋白、白蛋白、碱性磷酸酶、γ-谷氨酰转移酶、血红蛋白浓度。使用酶联免疫吸附测定法测定血清IL-41的水平。采用多因素Logistic回归分析确定与HUA相关的独立危险因素，并应用受试者工作特征（ROC）曲线分析IL-41的临床诊断价值。我们使用 Spearman 等级相关性评估了 IL-41 与临床参数之间的关联。

结果

HUA 患者的循环 IL-41 水平显着高于 HC 组（分别为 460.3 pg/mL 和 261.3 pg/mL；P  < 0.001）。HUA 患者中 IL-41 的 ROC 曲线下面积 (AUC) 为 0.7443（截止值为 311.055 pg/mL，敏感性为 68.18%，特异性为 72.73%），而 IL-41 的 AUC 为 0.7443（截止值为 311.055 pg/mL，敏感性为 68.18%，特异性为 72.73%） -41结合血小板计数为0.8109。相关分析显示HCs和HUA患者循环IL-41水平与年龄呈正相关。

结论

我们在此证明 HUA 患者血清 IL-41 升高，并且它可能构成与 HUA 相关的抗炎反应的新生物标志物。