It has recently been shown that the administration of probiotics can modulate the microbiota-gut-brain axis and may have favorable effects in models of Parkinson’s disease. In this study, we used a hemiparkinsonism model induced by the neurotoxin 6-OHDA to evaluate the efficacy of the administration of a four-week administration of a mixture containing the microorganisms Lactobacillus fermentum LH01, Lactobacillus reuteri LH03, and Lactobacillus plantarum LH05. The hemiparkinsonism model induced an increase in rotations in the apomorphine test, along with a decrease in the latency time to fall in the rotarod test on days 14 and 21 after surgery, respectively. The administration of probiotics was sufficient to improve this condition. The model also showed a decrease in tyrosine hydroxylase immunoreactivity in the striatum and the number of labeled cells in the substantia nigra, both of which were counteracted by the administration of probiotics. The permeability of the blood-brain barrier was increased in the model, but this effect was reversed by the probiotics for both brain regions. The gut barrier was permeated with the model, and this effect was reversed and dropped to lower levels than the control group after the administration of probiotics. Finally, lipid peroxidation showed a pattern of differences similar to that of permeabilities. The inhibition of the permeability of the blood-brain and gut barriers mediated by the administration of probiotics will likely provide protection by downregulating oxidative stress, thus affecting the rotarod test performance.

中文翻译：

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口服乳酸菌可抑制帕金森病模型中血脑和肠屏障的通透性

最近的研究表明，服用益生菌可以调节微生物群-肠-脑轴，并可能对帕金森病模型产生有利的影响。在本研究中，我们使用神经毒素6-OHDA诱导的偏侧帕金森病模型来评估含有微生物发酵乳杆菌LH01、罗伊氏乳杆菌LH03和植物乳杆菌LH05的混合物的为期4周的给药效果。偏侧帕金森病模型分别在术后第 14 天和第 21 天引起阿朴吗啡测试中旋转次数的增加，以及旋转棒测试中跌倒潜伏时间的缩短。服用益生菌足以改善这种状况。该模型还显示纹状体中酪氨酸羟化酶免疫反应性和黑质中标记细胞数量的减少，这两种情况都可以通过服用益生菌来抵消。在模型中，血脑屏障的通透性有所增加，但益生菌对两个大脑区域都逆转了这种效应。肠道屏障被模型渗透，在给予益生菌后，这种效应被逆转并降至低于对照组的水平。最后，脂质过氧化显示出与渗透性相似的差异模式。服用益生菌介导的血脑和肠道屏障渗透性的抑制可能会通过下调氧化应激提供保护，从而影响转棒测试的表现。