Recently, we discovered two novel orthobornaviruses in colubrid and viperid snakes using an in silico data mining approach. Here, we present the results of a screening of more than 100,000 nucleic acid sequence datasets of fish samples from the Sequence Read Archive (SRA) for potential bornaviral sequences. We discovered the potentially complete genomes of seven bornavirids in datasets from osteichthyans and chondrichthyans. Four of these are likely to represent novel species within the genus Cultervirus, and we propose that one genome represents a novel genus within the family of Bornaviridae. Specifically, we identified sequences of Wǔhàn sharpbelly bornavirus (WhSBV) in sequence data from the widely used grass carp liver and kidney cell lines L8824 and CIK, respectively. A complete genome of Murray-Darling carp bornavirus (MDCBV) was identified in sequence data from a goldfish (Carassius auratus). The newly discovered little skate bornavirus (LSBV), identified in the little skate (Leucoraja erinacea) dataset, contained a novel and unusual genomic architecture (N-Vp1-Vp2-X-P-G-M-L), as compared to other bornavirids. Its genome is thought to encode two additional open reading frames (tentatively named Vp1 and Vp2), which appear to represent ancient duplications of the gene encoding the viral glycoprotein (G). The datasets also provided insights into the possible transcriptional gradients of these bornavirids and revealed previously unknown splicing mechanisms.

中文翻译：

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深入研究鱼类博尔纳病毒：通过综合数据挖掘揭示隐藏的多样性和转录策略

最近，我们使用计算机数据挖掘方法在游蛇和蝰蛇中发现了两种新型正博尔纳病毒。在这里，我们展示了对来自序列读取档案 (SRA) 的 100,000 多个鱼类样本核酸序列数据集进行筛选的结果，以寻找潜在的博尔病毒序列。我们在骨鱼和软骨鱼的数据集中发现了七种博纳病毒科的潜在完整基因组。其中四个可能代表了 Cultervirus 属中的新物种，我们提出一个基因组代表了 Bornaviridae 家族中的一个新属。具体来说，我们分别从广泛使用的草鱼肝脏和肾细胞系 L8824 和 CIK 的序列数据中鉴定出武汉尖腹博纳病毒 (WhSBV) 的序列。从金鱼（Carassius auratus）的序列数据中鉴定出墨累-达令鲤鱼病毒（MDCBV）的完整基因组。在小鳐鱼 (Leucoraja erinacea) 数据集中发现的新发现的小鳐鱼博尔纳病毒 (LSBV)，与其他博尔纳病毒科相比，包含新颖且不寻常的基因组结构 (N-Vp1-Vp2-XPGML)。它的基因组被认为编码两个额外的开放阅读框（暂定名为Vp1和Vp2），这似乎代表了编码病毒糖蛋白（G）的基因的古老重复。这些数据集还提供了对这些博纳病毒科可能的转录梯度的见解，并揭示了以前未知的剪接机制。