Objective

Pteropyrum scoparium Jaub. & Spach locally known as “Sidaf” is a meal known to the ancient Omani people with many health benefits. It is traditionally used in Oman to treat high cholesterol, hypertension, indigestion problems, wound healing, and diabetes. However, these claims are yet to be scientifically proven. Hence, this study aimed to perform phytochemical, antioxidant, and antimicrobial analysis of P. scoparium leaves aqueous and alcoholic extracts to confirm its medicinal potential.

Methods

A detailed phytochemical analysis of ethanol and aqueous extracts of leaves was carried out to confirm the presence of bioactive substances. DPPH (2,2′-diphenyl-1-picrylhydrazyl), agar-well diffusion and disc diffusion methods were used to evaluate antioxidant and antimicrobial potential, respectively. The extracts were tested against four microorganisms viz. E. coli (ATCC 25922), S. aureus (ATCC 23235), Penicillium sp. (ATCC 11597) and Rhizopus stolonifer (ATCC 14037).

Results

The ethanol extract exhibited higher DPPH scavenging activity than aqueous extract that was confirmed with IC₅₀ values of both extracts. However, the aqueous extract was found to be significantly more effective as an antimicrobial agent than the ethanol extract. This could be due to higher coumarins content that is thrice as much as in ethanol extract. One-way repeated measure RM ANOVA showed that there was a statistically significant difference in the antimicrobial susceptibility of all four organisms for the aqueous and ethanol well diffusion extracts (DF = 7; SS = 56.350, MS = 8.050; F = 5.865; P < 0.001). The highest mean zone of inhibition was recorded for S. aureus (12 ± 3.851 mm) well diffusion aqueous extract followed by R. stolonifer (11.750 ± 4.250 mm) well diffusion aqueous extract, and S. aureus (10.625 ± 3.771 mm) well diffusion ethanol extract.

Conclusions

Phytochemical screening of ethanol and aqueous extracts revealed the presence of alkaloids, glycosides, carbohydrates, amino acid, fats & fixed oils, phenolic compounds & tannins, proteins, phytosterols, saponins, gum & mucilage, terpenoids, coumarins and anthocyanins. The findings from this study will be useful in evaluating the phytochemical constituents present in the extract and developing commercial drugs as antioxidant and antimicrobial agents based on this plant.

中文翻译：

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Pteropyum scoparium (Jaub. & Spach) Sidaf 粗提物的植物化学筛选及抗氧化和抗菌功效的体外评价

客观的

紫檀 (Pteropyrum scoparium) Jaub。& Spach 当地称为“Sidaf”，是古代阿曼人熟知的一种膳食，对健康有很多好处。它在阿曼传统上用于治疗高胆固醇、高血压、消化不良问题、伤口愈合和糖尿病。然而，这些说法尚未得到科学证实。因此，本研究旨在对P. scoparium叶水提取物和酒精提取物进行植物化学、抗氧化和抗菌分析​​，以确认其药用潜力。

方法

对乙醇和叶子的水提取物进行了详细的植物化学分析，以确认生物活性物质的存在。DPPH（2,2'-二苯基-1-三硝基苯肼）、琼脂井扩散法和纸片扩散法分别用于评估抗氧化和抗菌潜力。提取物针对四种微生物进行了测试，即。大肠杆菌(ATCC 25922) 、金黄色葡萄球菌(ATCC 23235)、青霉菌。（ATCC 11597）和匍匐根霉（ATCC 14037）。

结果

乙醇提取物表现出比水提取物更高的DPPH清除活性，这通过两种提取物的IC ₅₀值得到证实。然而，发现水提取物作为抗菌剂比乙醇提取物更有效。这可能是由于香豆素含量较高，是乙醇提取物的三倍。单向重复测量 RM 方差分析显示，所有四种生物体对水性和乙醇良好扩散提取物的抗菌敏感性存在统计学显着差异（DF = 7；SS = 56.350，MS = 8.050；F = 5.865；P < 0.001）。记录了金黄色葡萄球菌(12 ± 3.851 mm) 孔扩散水提取物的最高平均抑制区，其次是R. stolonifer (11.750 ± 4.250 mm) 孔扩散水提取物和金黄色葡萄球菌( 10.625 ± 3.771 mm)孔扩散水提取物乙醇提取物。

结论

乙醇和水提取物的植物化学筛选表明存在生物碱、糖苷、碳水化合物、氨基酸、脂肪和固定油、酚类化合物和单宁、蛋白质、植物甾醇、皂苷、树胶和粘液、萜类化合物、香豆素和花青素。这项研究的结果将有助于评估提取物中存在的植物化学成分，并开发基于该植物的抗氧化剂和抗菌剂商业药物。