Purpose

Lizard regeneration derives from the re-activation of a number of developmental genes after tail amputation. Among genes with the highest expression, as indicated from the transcriptome, is lix1 which functional role is not known.

Method

An antibody that cross-reacts with the lizard Podarcis muralis lix1 has been utilized to detect by immunofluorescence the sites of localization of the protein in the regenerating tail.

Results

Lix1-protein is almost exclusively localized in the regenerating spinal cord (ependyma) and nerves growing into the blastema, in sparse blastema cells but is undetectable in other tissues.

Conclusions

Since the spinal cord is essential to stimulate tail regeneration it is hypothesized that the lix1 protein is part of the signaling or growing factors produced from the regenerating spinal cord that are needed for tail regeneration of the lizard tail.

中文翻译：

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lix1在蜥蜴再生尾部的免疫定位表明该蛋白主要存在于神经组织中

目的

蜥蜴的再生源于断尾后许多发育基因的重新激活。从转录组中可以看出，在表达量最高的基因中，lix1的功能尚不清楚。

方法

一种与蜥蜴Podarcis muralis lix1 发生交叉反应的抗体已被用来通过免疫荧光检测该蛋白质在再生尾巴中的定位位点。

结果

Lix1-蛋白几乎完全位于再生脊髓（室管膜）和生长到胚基的神经中，在稀疏的胚基细胞中，但在其他组织中检测不到。

结论

由于脊髓对于刺激尾巴再生至关重要，因此假设 lix1 蛋白是再生脊髓产生的信号或生长因子的一部分，这些因子是蜥蜴尾巴再生所需的。