Cellulose and Polymer-based substrates are promising for SERS detection because they can be produced on demand in different sizes and formats and allow detection on the surface of samples owing to their flexibility. The present study selected a substrate with high SERS performance by selecting the following experimental variables: Ag nanoparticles (AgNP) protecting layer, flexible support pretreatment, and support nature (cellulosic paper – hydrophilic or PTFE and PDMS–hydrophobic). It was observed that the citrate layer, as a protector for AgNP, showed significant interference from citrate ions in analyte detection. The cellulosic support-based substrate presented the best SERS performance when pretreated with KCl before the cationic dye. Finally, because of the larger concentration of nanoparticles on the surface, the polymeric supports performed better than the cellulosic ones, with RSD values of approximately 5% for the cationic dye; for the anionic dye, the RSD value obtained was 26%.

纤维素和聚合物基底在 SERS 检测中很有前景，因为它们可以根据需要生产不同尺寸和格式，并且由于其灵活性而允许在样品表面进行检测。本研究通过选择以下实验变量来选择具有高 SERS 性能的基底：银纳米粒子 (AgNP) 保护层、柔性支撑预处理和支撑性质（纤维素纸 - 亲水性或 PTFE 和 PDMS - 疏水性）。据观察，柠檬酸盐层作为 AgNP 的保护剂，在分析物检测中显示出柠檬酸盐离子的显着干扰。当在阳离子染料之前用 KCl 进行预处理时，基于纤维素支撑的基底呈现出最佳的 SERS 性能。最后，由于表面纳米粒子浓度较高，聚合物载体的性能优于纤维素载体，阳离子染料的 RSD 值约为 5%；对于阴离子染料，获得的RSD值为26%。