Thymus satureioides Cosson is an endemic medicinal and aromatic plant species of both Morocco and Algeria, holds significant cultural and economic importance. It is used in traditional medicine to treat various diseases and also as a culinary spice herb. T. satureioides has become a key export for Morocco. However, increasing market demand and the effects of climate change have led to a decline in its wild populations, raising concerns about the scarcity of this valuable natural resource. To address these challenges, a comprehensive understanding of the environmental requirements for the germination of T. satureioides is essential for planning and prioritizing conservation efforts in its native habitats. In this context, the aim of this study was to assess the combined effects of various pretreatments and temperature and of salinity, drought stress, and gibberellin on the germination parameters of T. satureioides. Seeds were collected from a wild plant population in the Anti-Atlas Mountains. The tests included five pretreatments (mechanical scarification, sulfuric acid (95%), dry heat, boiling water and hydrogen peroxide) in conjunction with five temperature conditions (10, 15, 20, 25 and 30 °C). Additionally, six concentrations of sodium chloride (0, 25, 50, 100, 150 and a 200 mM), six water potential levels of polyethylene glycol (PEG-6000) (0, −0.07, −0.14, −0.22, −0.32 and - 0.53 MPa) and six concentrations of gibberellin (0, 50, 100, 500, 1000 and 2000 ppm) were tested. Results showed that seeds can germinate without any treatment. However, seeds treated with dry heat followed by incubation at 20 °C improved all the germination parameters. In addition, germination parameters progressively decreased and delayed with increasing levels of both salt and water stress. Without stress, the germination percentage was 100%, with increasing salt stress to 200 mM germination was completely inhibited. Although, seeds showed a low germination percentage (5,67%) at the highest osmotic potential (−0.53 MPa).

百里香 (Thymus satureioides Cosson) 是摩洛哥和阿尔及利亚的特有药用和芳香植物物种，具有重要的文化和经济重要性。它在传统医学中用于治疗各种疾病，也用作烹饪香料草药。T. satureioides 已成为摩洛哥的主要出口产品。然而，市场需求的增加和气候变化的影响导致其野生种群数量减少，引发人们对这种宝贵自然资源稀缺性的担忧。为了应对这些挑战，全面了解 T. satureioides 发芽的环境要求对于规划和优先考虑其原生栖息地的保护工作至关重要。在此背景下，本研究的目的是评估各种预处理和温度以及盐度、干旱胁迫和赤霉素对 T. satureioides 发芽参数的综合影响。种子是从安提阿特拉斯山脉的野生植物群中收集的。测试包括五种预处理（机械划痕、硫酸(95%)、干热、沸水和过氧化氢）以及五种温度条件（10、15、20、25 和 30 °C）。此外，还有六种浓度的氯化钠（0、25、50、100、150 和 200 mM）、六种水势水平的聚乙二醇 (PEG-6000)（0、-0.07、-0.14、-0.22、-0.32 和- 0.53 MPa）和六种浓度的赤霉素（0、50、100、500、1000 和 2000 ppm）进行了测试。结果表明，种子无需任何处理即可发芽。然而，经过干热处理并在 20°C 下孵育的种子改善了所有发芽参数。此外，随着盐和水胁迫水平的增加，发芽参数逐渐降低和延迟。在没有胁迫的情况下，发芽率为100%，当盐胁迫增加到200 mM时，发芽率被完全抑制。尽管如此，种子在最高渗透势（-0.53 MPa）下的发芽率较低（5.67%）。