Pseudomonas simiae WCS417 is a plant growth-promoting rhizobacterium that improves plant health and development. In this study, we investigate the early leaf responses of Arabidopsis thaliana to WCS417 exposure and the possible involvement of formate dehydrogenase (FDH) in such responses. In vitro-grown A. thaliana seedlings expressing an FDH::GUS reporter show a significant increase in FDH promoter activity in their roots and shoots after 7 days of indirect exposure (without contact) to WCS417. After root exposure to WCS417, the leaves of FDH::GUS plants grown in the soil also show an increased FDH promoter activity in hydathodes. To elucidate early foliar responses to WCS417 as well as FDH involvement, the roots of A. thaliana wild-type Col and atfdh1-5 knock-out mutant plants grown in soil were exposed to WCS417, and proteins from rosette leaves were subjected to proteomic analysis. The results reveal that chloroplasts, in particular several components of the photosystems PSI and PSII, as well as members of the glutathione S-transferase family, are among the early targets of the metabolic changes induced by WCS417. Taken together, the alterations in the foliar proteome, as observed in the atfdh1-5 mutant, especially after exposure to WCS417 and involving stress-responsive genes, suggest that FDH is a node in the early events triggered by the interactions between A. thaliana and the rhizobacterium WCS417. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

中文翻译：

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拟南芥早期叶蛋白质组对根暴露于根际假单胞菌 WCS417 的响应。

西米亚假单胞菌 WCS417 是一种促进植物生长的根际细菌，可改善植物健康和发育。在本研究中，我们研究了拟南芥对 WCS417 暴露的早期叶片反应以及甲酸脱氢酶 (FDH) 可能参与此类反应。表达 FDH::GUS 报告基因的体外培养拟南芥幼苗在间接暴露（无接触）WCS417 7 天后，其根和芽中的 FDH 启动子活性显着增加。根部暴露于 WCS417 后，在土壤中生长的 FDH::GUS 植物的叶子也显示出水囊中 FDH 启动子活性的增加。为了阐明早期叶对 WCS417 的反应以及 FDH 的参与，将在土壤中生长的拟南芥野生型 Col 和 atfdh1-5 敲除突变体植物的根暴露于 WCS417，并对来自莲座叶的蛋白质进行蛋白质组分析。结果表明，叶绿体，特别是光系统 PSI 和 PSII 的几个组成部分，以及谷胱甘肽 S-转移酶家族的成员，是 WCS417 诱导的代谢变化的早期目标之一。总而言之，在 atfdh1-5 突变体中观察到的叶蛋白质组的变化，特别是在暴露于 WCS417 并涉及应激反应基因之后，表明 FDH 是由拟南芥和拟南芥之间的相互作用触发的早期事件的一个节点。根际细菌WCS417。[公式：见文字] 版权所有 © 2023 作者。这是一篇根据 CC BY-NC-ND 4.0 国际许可证分发的开放获取文章。